Human EDAR Antibody

Catalog # Availability Size / Price Qty
AF157
AF157-SP
Product Details
Citations (1)
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Human EDAR Antibody Summary

Species Reactivity
Human
Specificity
Detects human EDAR in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhCD40, rhDR6, rhTAJ, rhTNF RI, rhTNF RII, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGFR, rhOPG and rhRANK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human EDAR
Glu27-Ile189
Accession # Q9UNE0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EDAR Fc Chimera (Catalog # 157-ER)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 4-16 µg/mL of this antibody will block 50% of the binding of 10 μg/mL of Recombinant Human EDA-A1 (Catalog # 3944-ED) to immobilized Recombinant Human EDAR Fc Chimera (Catalog # 157-ER) coated at 1 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region; the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Human EDAR is a 488 amino acid (aa) protein with a predicted 26 aa signal, a 163 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The human and mouse EDAR homologs share 91% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2, which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References
  1. Headon, D.J. and P.A. Overbeek (1999) Nat.Genet. 22:370.
  2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
  3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
  4. Schneider, P. et al. (2001) J. Biol. Chem. 276:18819.
  5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
  6. Yan, M. et al. (2000) Science 290:523.
Long Name
Ectodysplasin Receptor
Entrez Gene IDs
10913 (Human); 13608 (Mouse); 102120238 (Cynomolgus Monkey)
Alternate Names
Anhidrotic ectodysplasin receptor 1; DLED3; Ectodermal dysplasia receptor; ectodysplasin 1, anhidrotic receptor; ectodysplasin A receptor; Ectodysplasin-A receptor; ED1R; ED5; EDA1R; EDA3EDA-A1 receptor; EDA-A1R; EDAR; FLJ94390; HRM1; mouse, homolog of; tumor necrosis factor receptor superfamily member EDAR

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Citation for Human EDAR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Functional Study of Ectodysplasin-A Mutations Causing Non-Syndromic Tooth Agenesis
    PLoS ONE, 2016;11(5):e0154884.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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