EDNRA (endothelin A receptor) is a 427 amino acid (aa) protein of the rhodopsin-like 7-transmembrane receptor family. It can be variably modified by N-linked glycoproteins, phosphorylation and palmitoylation. It binds endothelins ET-1 and ET-2 preferentially, while EDNRB binds all three forms equally. Developmentally, EDNRA is expressed by cranial neural crest cells and induces facial morphogenesis. It is expressed by adult blood vessel smooth muscle cells, and is primarily responsible for the vasoconstrictor effects of ET-1. Human EDNRA shares 86% aa identity with mouse and rat EDNRA within the combined extracellular portions of the molecule.
Human EDNRA/Endothelin R Type A Antibody
R&D Systems | Catalog # MAB65381
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 485709
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human EDNRA/Endothelin R Type A
Accession # NP_001948
Accession # NP_001948
Specificity
Detects human EDNRA/Endothelin R Type A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human EDNRA/Endothelin R Type A Antibody
Detection of EDNRA in HEK293 Human Cell Line Transfected with Human EDNRA and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human EDNRA or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human EDNRA Monoclonal Antibody (Catalog # MAB65381) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.Applications for Human EDNRA/Endothelin R Type A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human EDNRA and eGFP
Sample: HEK293 Human Cell Line Transfected with Human EDNRA and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: EDNRA/Endothelin R Type A
Long Name
Endothelin Receptor Type A
Alternate Names
EDNRA, ETAR, ETRA
Gene Symbol
EDNRA
UniProt
Additional EDNRA/Endothelin R Type A Products
Product Documents for Human EDNRA/Endothelin R Type A Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human EDNRA/Endothelin R Type A Antibody
For research use only
Related Research Areas
Citations for Human EDNRA/Endothelin R Type A Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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