Human EDNRA/Endothelin R Type A Antibody

Catalog # Availability Size / Price Qty
MAB65381-100
MAB65381-SP
Human EDNRA/Endothelin R Type A Antibody in Flow Cytometry
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Human EDNRA/Endothelin R Type A Antibody Summary

Species Reactivity
Human
Specificity
Detects human EDNRA/Endothelin R Type A in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 485709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human EDNRA/Endothelin R Type A
Accession # NP_001948
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of EDNRA in HEK293 Human Cell Line Transfected with Human EDNRA and eGFP by Flow Cytometry. View Larger

Detection of EDNRA in HEK293 Human Cell Line Transfected with Human EDNRA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human EDNRA or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human EDNRA Monoclonal Antibody (Catalog # MAB65381) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EDNRA/Endothelin R Type A

EDNRA (endothelin A receptor) is a 427 amino acid (aa) protein of the rhodopsin-like 7-transmembrane receptor family. It can be variably modified by N-linked glycoproteins, phosphorylation and palmitoylation.  It binds endothelins ET-1 and ET-2 preferentially, while EDNRB binds all three forms equally. Developmentally, EDNRA is expressed by cranial neural crest cells and induces facial morphogenesis. It is expressed by adult blood vessel smooth muscle cells, and is primarily responsible for the vasoconstrictor effects of ET-1. Human EDNRA shares 86% aa identity with mouse and rat EDNRA within the combined extracellular portions of the molecule.

Long Name
Endothelin Receptor Type A
Entrez Gene IDs
1909 (Human); 13617 (Mouse)
Alternate Names
EDNRA; endothelin receptor type A; endothelin-1 receptor; endothelin-1-specific receptor; ET1-specific type; ETAR; ETRA; G protein-coupled receptor; hET-AR

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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