Human eIF4B Antibody

Catalog # Availability Size / Price Qty
AF3800
AF3800-SP
Detection of Human eIF4B by Western Blot.
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Human eIF4B Antibody Summary

Species Reactivity
Human
Specificity
Detects human and mouse eIF4B in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human eIF4B
Met1-Arg200
Accession # P23588
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human eIF4B antibody by Western Blot. View Larger

Detection of Human eIF4B by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human eIF4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF4B at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF4B

eIF4B (eukaryotic translation initiation factor 4B),an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with eIF4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF4E.

Long Name
Eukaryotic Translation Initiation Factor 4B
Entrez Gene IDs
1975 (Human); 75705 (Mouse); 300253 (Rat)
Alternate Names
Eif4a2; eIF4B; EIF-4B; eukaryotic translation initiation factor 4B; PRO1843

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human eIF4B Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human