|Detection of Human eIF4B by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and Neuro‑2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human eIF4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3800) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eIF4B at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
eIF4B (eukaryotic translation initiation factor 4B),an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with eIF4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF4E.
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