Human Endoglin/CD105 Biotinylated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Endoglin in ELISAs and Western blots. In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant mouse Endoglin, recombinant human (rh) Activin A, and rhTGF‑ beta 1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Endoglin
    Glu26-Gly256
    Accession # Q5T9B9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Endoglin/CD105 (Catalog # 1097-EN)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Human Endoglin/CD105 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human Endoglin/CD105 Antibody (Catalog # MAB10972)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Endoglin/CD105 Biotinylated Antibody (Catalog # BAF1097)
  • ELISA Standard
     
    Recombinant Human Endoglin/CD105 Protein (Catalog # 1097-EN)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Endoglin/CD105 in U937 Human Cell Line. Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557‑conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human Endoglin/CD105 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2), but only after binding T beta RII (8). Similarly, they interact with Activin A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity (15).

  • References:
    1. Gougos, A. and M. Letarte (1990) J. Biol. Chem. 265:8361.
    2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
    3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
    4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
    5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
    6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
    7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
    8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
    9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
    10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
    11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
    12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
    13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
    14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
    15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
  • Entrez Gene IDs:
    2022 (Human); 13805 (Mouse); 497010 (Rat)
  • Alternate Names:
    CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1
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