Human Endoglin/CD105 Antibody
Human Endoglin/CD105 Antibody Summary
Glu26-Gly586
Accession # Q5T9B9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Endoglin/CD105 by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Endoglin/CD105 in U937 Human Cell Line. Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
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Endoglin/CD105 in Mouse Kidney. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human Endoglin/CD105 by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) was used at 20 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Western Blot Shows Human Endoglin/CD105 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglin/CD105
Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and Betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 amino acid (aa) signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to antiangiogenic activity (15).
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- Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
- Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
- Moody, J.L. et al. (2007) Stem Cells 25:2809.
- Velasco, S. et al. (2008) J. Cell Sci. 121:913.
- Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
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- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
- Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
- Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
- Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
- Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.
- Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Product Datasheets
Citations for Human Endoglin/CD105 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Endoglin Is an Important Mediator in the Final Common Pathway of Chronic Kidney Disease to End-Stage Renal Disease
Authors: T Gerrits, IJ Brouwer, KL Dijkstra, R Wolterbeek, JA Bruijn, M Scharpfene, HJ Baelde
International Journal of Molecular Sciences, 2022;24(1):.
Species: Human
Sample Types: Protein Lysates
Applications: Western Blot -
Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes
Authors: PL Mendez, L Obendorf, J Jatzlau, W Burdzinski, M Reichenbac, V Nageswaran, A Haghikia, V Stangl, C Hiepen, P Knaus
Bmc Biology, 2022;20(1):210.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia
Authors: DN Mitroi, M Tian, R Kawaguchi, WE Lowry, ST Carmichael
Journal of Cellular and Molecular Medicine, 2022;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation
Authors: KJG Kenswil, P Pisterzi, G Sánchez-Du, C van Dijk, A Lolli, C Knuth, B Vanchin, AC Jaramillo, RM Hoogenboez, MA Sanders, J Feyen, T Cupedo, IG Costa, R Li, EMJ Bindels, K Lodder, B Blom, PK Bos, MJ Goumans, P Ten Dijke, E Farrell, G Krenning, MHGP Raaijmaker
Cell Stem Cell, 2021;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Reversing pathology in a preclinical model of Alzheimer's disease by hacking cerebrovascular neoangiogenesis with advanced cancer therapeutics
Authors: CSB Singh, KB Choi, L Munro, HY Wang, CG Pfeifer, WA Jefferies
EBioMedicine, 2021;71(0):103503.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering
Authors: JV Korpershoe, M Rikkers, TS de Windt, MA Tryfonidou, DBF Saris, LA Vonk
International Journal of Molecular Sciences, 2021;22(16):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1
Authors: G Galaris, K Montagne, JH Thalgott, GJPE Goujon, S van den Dr, S Martin, HJ Mager, CL Mummery, TJ Rabelink, F Lebrin
International Journal of Molecular Sciences, 2021;22(16):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Endoglin Promotes Myofibroblast Differentiation and Extracellular Matrix Production in Diabetic Nephropathy
Authors: T Gerrits, M Zandbergen, R Wolterbeek, JA Bruijn, HJ Baelde, M Scharpfene
Int J Mol Sci, 2020;21(20):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression
Authors: Q Zhang, DY Yi, BZ Xue, WW Wen, YP Lu, A Abdelmakso, MX Sun, DT Yuan, HY Zhao, NX Xiong, W Xiang, P Fu
Cell Death Dis, 2018;9(11):1101.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
Nat. Cell Biol., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression
Authors: ML García-Her, NO Uribe-Urib, R Espinosa-G, WM Kast, SA Khader, J Rangel-Mor
Front Immunol, 2017;8(0):563.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers
Authors: HF Moffett, ME Coon, S Radtke, SB Stephan, L McKnight, A Lambert, BL Stoddard, HP Kiem, MT Stephan
Nat Commun, 2017;8(1):389.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension
PLoS ONE, 2016;11(9):e0162144.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
Sci Rep, 2016;6(0):32382.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy
Invest Ophthalmol Vis Sci, 2016;57(11):5031-5037.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Umbilical cord mesenchymal stromal cells affected by gestational diabetes mellitus display premature aging and mitochondrial dysfunction.
Authors: Kim J, Piao Y, Pak Y, Chung D, Han Y, Hong J, Jun E, Shim J, Choi J, Kim C
Stem Cells Dev, 2015;24(5):575-86.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
PLoS ONE, 2015;10(3):e0121421.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Authors: Riordan D, Varma S, West R, Brown P
PLoS ONE, 2015;10(7):e0128975.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study.
Authors: Boonstra M, Verbeek F, Mazar A, Prevoo H, Kuppen P, van de Velde C, Vahrmeijer A, Sier C
BMC Cancer, 2014;14(0):269.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.
Authors: Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
PLoS ONE, 2014;9(6):e100310.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues.
Authors: Barsoum IB, Renaud SJ, Graham CH
Am. J. Pathol., 2011;178(6):2888-96.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Glial cell line-derived neurotrophic factor-secreting genetically modified human bone marrow-derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease.
Authors: Glavaski-Joksimovic A, Virag T, Mangatu TA
J. Neurosci. Res., 2010;88(12):2669-81.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Lysates and supernatants of pancreatic cancer cells AsPC-1 and MIA PaCa-2 were examined under denaturing/reducing conditions by SDS-PAGE/western blots and subsequent antibody stainings. Nitrocellulose membrane was probed with 0.13 ug/ml. This antibody stains endoglin as expected.
