Human Endomucin Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Endomucin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Endomucin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Endomucin
    Asn19-Gly139
    Accession # Q9ULC0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Endomucin by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7206) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Endomucin at approximately 80 to 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endomucin

Endomucin (Endothelial sialomucin; also MUC-14, Endomucin-2 and Ga34) is an 85-115 kDa glycoprotein that belongs to no known family of molecules. It is expressed by primitive bone marrow stem cells, endothelial cells of both blood and lymphatic vessels, and select keratinocytes. Endomucin appears to create an environment that discourages leukocyte binding to vascular walls, and yet may also serve as an L-Selectin ligand. Mature human Endomucin is a 243 amino acid (aa) type I transmembrane glycoprotein (aa 19-261). It contains a 172 aa extracellular domain (ECD) (aa 19-190) plus a 50 aa cytoplasmic tail (aa 212-261). The ECD possesses a uteroglobin-like domain (aa 19-74) plus multiple sites for N- and O-linked glycosylation. There are potential splice variants that seem to parallel those in mouse. One shows a deletion of aa 140-222 and is likely soluble, while another possesses a Ser substitution for aa 126-139. Over aa 19-139, human Endomucin shares only 35% aa identity with mouse Endomucin.

  • Entrez Gene IDs:
    51705 (Human); 59308 (Mouse); 295490 (Rat)
  • Alternate Names:
    EMCN; EMCN2; Endomucin; MUC-14
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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