Detection of Human Endomucin by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Endomucin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7206) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Endomucin at approximately 80 to 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Endomucin (Endothelial sialomucin; also MUC-14, Endomucin-2 and Ga34) is an 85-115 kDa glycoprotein that belongs to no known family of molecules. It is expressed by primitive bone marrow stem cells, endothelial cells of both blood and lymphatic vessels, and select keratinocytes. Endomucin appears to create an environment that discourages leukocyte binding to vascular walls, and yet may also serve as an L-Selectin ligand. Mature human Endomucin is a 243 amino acid (aa) type I transmembrane glycoprotein (aa 19-261). It contains a 172 aa extracellular domain (ECD) (aa 19-190) plus a 50 aa cytoplasmic tail (aa 212-261). The ECD possesses a uteroglobin-like domain (aa 19-74) plus multiple sites for N- and O-linked glycosylation. There are potential splice variants that seem to parallel those in mouse. One shows a deletion of aa 140-222 and is likely soluble, while another possesses a Ser substitution for aa 126-139. Over aa 19-139, human Endomucin shares only 35% aa identity with mouse Endomucin.
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