Human EphA1 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human EphA1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) EphA3, rmEphA8, rmEphA4, recombinant rat (rr) EphA5, rmEphA2, rmEphA6, and rmEphA7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EphA1
    Lys26-Glu547
    Accession # AAD43440
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    MCF-7 human breast cancer cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human EphA1 by Western Blot. Western blot shows lysates of T47D human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EphA1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
EphA1 in MCF‑7 Human Cell Line. EphA1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human EphA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF638) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EphA1

EphA1, also known as Eph and Esk (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA1 has been shown to bind ephrin-A1 (2, 3). The extracellular domains of mouse and human EphA1 share greater than 91% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  • References:
    1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
    2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
    3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
  • Entrez Gene IDs:
    2041 (Human); 13835 (Mouse)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.1; EPH receptor A1; eph tyrosine kinase 1; EphA1; EPHephrin type-A receptor 1; EPHT; EPHT1erythropoietin-producing hepatoma amplified sequence; Esk; MGC163163; oncogene EPH; soluble EPHA1 variant 1; soluble EPHA1 variant 2; Tyrosine-protein kinase receptor EPH
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Alteration of the EphA2/Ephrin-A signaling axis in psoriatic epidermis.
    Authors: Gordon K, Kochkodan J, Blatt H, Lin S, Kaplan N, Johnston A, Swindell W, Hoover P, Schlosser B, Elder J, Gudjonsson J, Getsios S
    J Invest Dermatol, 2013;133(3):712-22.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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Chicken Anti-Goat IgG Biotinylated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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