Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ephrin-B3 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Ephrin-B3
Leu28-Ser224
Accession # Q15768

Specificity

Detects human Ephrin-B3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Ephrin-A5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Ephrin‑B3 Antibody

Detection of Mouse and Rat Ephrin‑B3 antibody by Western Blot.

Detection of Mouse and Rat Ephrin‑B3 by Western Blot.

Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Ephrin-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF395) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Ephrin-B3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Ephrin‑B3 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of rat embryo (E15) and rat brain

Western Blot

1 µg/mL
Sample: Mouse brain tissue and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-B3

Ephrin-B3, also known as NLERK-2, Elk-L3, EFL-6, ELF-3 and LERK-8 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can be tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 aa long and are highly conserved, especially the last 33 aa. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B3 has been shown to bind EphA4, EphB1, EphB2, and EphB3 (2, 3). The extracellular domains of murine and human Ephrin-B3 share 98% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter]. Cell (1997) 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

EFL-6, Efnb3, Elk-L3, EphrinB3, Epl8, LERK-8, NLERK-2

Entrez Gene IDs

1949 (Human); 13643 (Mouse)

Gene Symbol

EFNB3

UniProt

Q15768

Additional Ephrin-B3 Products

Product Documents for Human Ephrin‑B3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Ephrin‑B3 Antibody

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Human Ephrin‑B3 Antibody

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Protocols

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