Estrogen-related receptor alpha (ERR-alpha, ERR1, ESRL1; NR3B1) is a member of the orphan nuclear receptor family. ERR-alpha is expressed in the liver, kidney, brain, heart, uterus, vagina and cervix. ERR-alpha takes part in the cell cycle and the fatty acid metabolizing of the musculus skeleti. ERR-alpha was shown to interact with ER-alpha. ERR-beta (ERR2; NR3B2) is also expressed in the liver, brain, uterus, vagina, and cervix. ERR-beta has a role in early placental development. ERR-gamma (ESRRG; NR3B3) is expressed in the brain, kidney, testis, lung, adrenal gland, pancreas, placenta and bone marrow. ERR-gamma was shown to be located in the critical region for Ushers syndrome.
Human ERR alpha 1/NR3B1 Antibody (Clone H5844)
R&D Systems | Catalog # PP-H5844-00
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, Direct ELISA, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # H5844
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Product Specifications
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human ERR alpha 1/NR3B1 Antibody (Clone H5844)
Application
Recommended Usage
Direct ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human myocardium
Sample: Immersion fixed paraffin-embedded sections of human myocardium
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using PP-H5844-00 in the following applications:
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: ERR alpha/NR3B1
Long Name
Estrogen-related Receptor alpha
Alternate Names
ERR1, ESRL1, ESRRA, NR3B1
Entrez Gene IDs
2101 (Human)
Gene Symbol
ESRRA
Additional ERR alpha/NR3B1 Products
Product Documents for Human ERR alpha 1/NR3B1 Antibody (Clone H5844)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human ERR alpha 1/NR3B1 Antibody (Clone H5844)
For research use only
Related Research Areas
Citations for Human ERR alpha 1/NR3B1 Antibody (Clone H5844)
Customer Reviews for Human ERR alpha 1/NR3B1 Antibody (Clone H5844) (1)
5 out of 5
1 Customer Rating
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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