Human FACA/FANCA Antibody Summary
Accession # O15360
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FACA/FANCA by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human FACA/FANCA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6026) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FACA/FANCA at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
FACA (Fanconi anemia group A) is a 160‑165 kDa protein that belongs to Fanconi anemia protein group I. FACA is widely expressed, activated by phosphorylation, partners with FANCG, and participates in the formation of an eight subunit Fanconi anemia core complex that ubiquitinates FANCD2 and FANCI. Ultimately, this contributes to the reactivation of stalled DNA repair replication forks. Human FACA is 1455 amino acids (aa) in length. It contains one NLS (aa 18‑34) and four phosphorylation sites at Ser849, 850, 858 and 1449. There are four potential splice variants. One shows a premature truncation after Cys297, a second contains a six aa substitution for aa 297‑1455, a third shows a deletion of aa 143‑174 accompanied by truncation after Cys297, and a fourth exhibits an alternative start site at Met528, accompanied by a 35 aa substitution for aa 1390‑1455. Over aa 35‑142, human FACA shares 61% aa identity with mouse FACA.
Citation for Human FACA/FANCA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Genomic amplification of Fanconi anemia complementation group A (FancA) in head and neck squamous cell carcinoma (HNSCC): Cellular mechanisms of radioresistance and clinical relevance
Authors: Kirsten Lauber
Cancer Lett., 2016;386(0):87-99.
Sample Types: Protein
Applications: Western Blot
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