Human Fatty Acid Synthase/FASN Antibody Summary
Accession # P49327
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Fatty Acid Synthase/FASN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western Blot Shows Human Fatty Acid Synthase/FASN Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293 human embryonic kidney parental cell line and Fatty Acid Synthase/FASN knockout HEK293 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fatty Acid Synthase/FASN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fatty Acid Synthase/FASN at approximately 275 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in knockout HEK293 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fatty Acid Synthase/FASN
FASN (Fatty Acid SyNthase; also FAS) is a cytosolic member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length. It contains multiple domains, including a beta ‑ketoacyl synthase domain (aa 1‑414), an acyl and malonyl transferase domain (aa 429‑817), an enoyl reductase domain (aa 1563‑1863), a beta ‑ketoacyl reductase domain (aa 1864‑2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 10‑212, human FASN shares 92% aa identity with mouse FASN.
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