Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Canine

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 190723
View all Fc gamma RII/CD32 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB
Ala46-Pro217
Accession # P31994

Specificity

Detects human Fc gamma RIIA and human Fc gamma RIIB in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse Fc gamma  RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Fc gamma  RII/CD32 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood monocytes and granulocytes

Western Blot

1 µg/mL
Sample: Recombinant Human Fc gamma RIIA/CD32a (Catalog # 1330-CD)
Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 4 using MAB1330 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RII/CD32

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T cells, and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Entrez Gene IDs

2212 (Human); 14130 (Mouse)

Gene Symbol

FCGR2A

UniProt

P31994

Additional Fc gamma RII/CD32 Products

Product Documents for Human Fc gamma  RII/CD32 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fc gamma  RII/CD32 Antibody

For research use only

Citations for Human Fc gamma  RII/CD32 Antibody

Customer Reviews for Human Fc gamma  RII/CD32 Antibody (1)

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  • Human Fc gamma  RII/CD32 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 05/03/2017
    Human Fc gamma  RII/CD32 Antibody MAB1330

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