Human Fer Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fer in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 690318
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Fer
    Asn93-Ala302
    Accession # P16591
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Fer in Human Liver.

Fer was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fer Monoclonal Antibody (Catalog # MAB7229) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fer
FER [feline encephalitis virus (FES)-related kinase], also called Tyk3, is a widely expressed 822 amino acid (aa), 94 kDa intracellular non-receptor tyrosine kinase of the FES/FPS family. Human FER contains an FCH (microtubule/cytoskeleton interaction) domain (aa 1‑58), a coiled coil oligomerization region, an SH2 domain (aa 460‑550) and a kinase domain (aa 563‑816). FER is found both in the cytoplasm and the nucleus, and interacts with several transmembrane receptors, cytosolic proteins, and nuclear chromatin. Through its interactions and kinase activity, Tyk3/FER influences synapse formation, cytoskeletal rearrangement, cell survival and cell cycle progression. Within the region used as an immunogen, human Tyk3/FER shares 97% aa sequence identity with mouse Tyk3/FER. Potential human Tyk3/FER isoforms truncated at aa 308 and 478 have been described.
  • Long Name:
    Feline Encephalitis Virus-related Kinase
  • Entrez Gene IDs:
    2241 (Human); 14158 (Mouse); 301737 (Rat)
  • Alternate Names:
    EC 2.7.10; EC 2.7.10.2; fer (fps/fes related) tyrosine kinase; Fer; p94-Fer; Phosphoprotein NCP94; Proto-oncogene c-Fer; TYK3; TYK3phosphoprotein NCP94; Tyrosine kinase 3; tyrosine-protein kinase Fer
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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