Human Ferroportin/SLC40A1 Antibody

MAB9924 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9924-100
MAB9924-SP

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Detection of Ferroportin/SLC40A1 in HEK293 Human Cell Line Transfected with Human Ferroportin/SLC40A1 and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Ferroportin/SLC40A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ferroportin/SLC40A1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1308C

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing human Ferroportin/SLC40A1
Accession # Q9NP59

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/mL

See below

Immunohistochemistry

1-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

Detection of Ferroportin/SLC40A1 antibody in HEK293 Human Cell Line Transfected with Human Ferroportin/SLC40A1 and eGFP antibody by Flow Cytometry.

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Detection of Ferroportin/SLC40A1 in HEK293 Human Cell Line Transfected with Human Ferroportin/SLC40A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human Ferroportin/SLC40A1 and eGFP was stained with either (A) Rabbit Anti-Human Ferroportin/SLC40A1 Monoclonal Antibody (Catalog # MAB9924) or (B) Rabbit IgG Isotype Control (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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Ferroportin/SLC40A1 in Human Liver. Ferroportin/SLC40A1 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Ferroportin/SLC40A1 Monoclonal Antibody (Catalog # MAB9924) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ferroportin/SLC40A1

Ferroportin-1 (FPN1), also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a multi-pass membrane protein that in humans is encoded by the SLC40A1 gene and is part of the Ferroportin (Fpn) Family. Ferroportin 1 is an iron-regulated transporter that is essential for iron homeostasis, playing a key role in intestinal iron absorption, as well as cellular iron release and efflux. Ferroportin can be regulated at many different levels including transcriptionally, post-transcriptionally, through mRNA stability and post-translationally, through protein turnover. Mutations affecting the SLC40A1 gene result in a disorder of iron metabolism characterized by iron overload. Excess iron is deposited in a variety of organs leading to their failure, and resulting in serious illnesses including cirrhosis, hepatomas, diabetes, cardiomyopathy and arthritis.

Long Name

Solute Carrier Family 40 Member 1

Entrez Gene IDs

30061 (Human); 53945 (Mouse); 170840 (Rat)

Alternate Names

Ferroportin; Ferroportin-1; FPN1; FPN1IREG1ferroportin 1; HFE4; HFE4ferroportin-1; IREG1; iron regulated gene 1; Iron-regulated transporter 1; member 3; MST079; MSTP079; MTP1; putative ferroportin 1 variant IIIB; SLC11A3; SLC11A3iron regulated gene 1; SLC40A1; solute carrier family 11 (proton-coupled divalent metal ion transporters); solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 40 (iron-regulated transporter), member 1; solute carrier family 40 member 1