Human FGF-23 Antibody

  (3 citations)
(1 Review)
    
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Product Details
Citations (3)
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  • Species Reactivity
    Human
  • Specificity
    Detects human FGF-23 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse FGF‑23 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FGF-23
    Tyr25-Ile251
    Accession # Q9GZV9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.24 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human FGF‑23 (Catalog # 2604-FG)
  • Neutralization
    Measured by its ability to neutralize FGF‑23-induced proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 1 µg/mL Recombinant Human FGF‑23, 5 µg/mL Recombinant Mouse Klotho, and 10 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Cell Proliferation Induced by FGF‑23 and Neutralization by Human FGF‑23 Antibody. In the presence of Recombinant Mouse Klotho (5 µg/mL, Catalog # 1819-KL) and heparin (10 µg/mL), Recombinant Human FGF‑23 (Catalog # 2604-FG) stimulates proliferation in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF‑23 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑23 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2604). The ND50 is typically 5‑15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF family, within a subfamily that also includes FGF-19 and FGF-21. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). FGF-19 subfamily members are highly diffusible molecules owing to their poor ECM/heparin sulfate binding and plasma-stabilizing intramolecular folds (2-4). Mature human FGF-23 contains an atypical (very low affinity) heparin binding site (aa 134‑162), a proteolytic cleavage site (Arg179-Ser180), and multiple O-linked glycosylation sites with Thr178 being of particular importance (4-7). O-linked glycosylation at Thr178 blocks the cleavage of FGF-23, thereby preventing loss of FGF-23 activity (7, 8). Mature human FGF-23 shows 72% aa identity to mouse FGF-23 and is active on mouse cells (6). FGF-23 exerts its effects through a ternary complex that includes Klotho and an FGF receptor (FGF R4 or the "c" isoforms of FGF R1 or FGF R3). Klotho has a restricted distribution that limits FGF-23 activity (9-11). FGF-23 is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone, and circulatory volume loading. It functions as an endocrine phosphatonin by suppressing circulating phosphate levels (12). FGF-23 interaction with renal proximal tubular epithelium decreases the renal resorption of phosphate by downregulating phosphate transporters and by suppressing vitamin D production. It also decreases the intestinal absorption of phosphate (13).

  • References:
    1. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    2. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
    3. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
    4. Harmer, N.J. et al. (2004) Biochemistry 43:629.
    5. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
    6. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
    7. Frishberg, Y. et al. (2007) J. Bone Miner. Res. 22:235.
    8. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
    9. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
    10. Urakawa, I. et al. (2006) Nature 444:770.
    11. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
    12. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
    13. Kurosu, H. et. al. (2007) J. Biol. Chem. 282:26687.
     
  • Long Name:
    Fibroblast Growth Factor 23
  • Entrez Gene IDs:
    8074 (Human); 64654 (Mouse)
  • Alternate Names:
    ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. FGF23 is synthesised locally by renal tubules and activates injury-primed fibroblasts
    Authors: ER Smith, SJ Tan, SG Holt, TD Hewitson
    Sci Rep, 2017;7(1):3345.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. FGF23 is endogenously phosphorylated in bone cells.
    Authors: Lindberg I, Pang H, Stains J, Clark D, Yang A, Bonewald L, Li K
    J Bone Miner Res, 2015;30(3):449-54.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
  3. FGF23 neutralization improves chronic kidney disease-associated hyperparathyroidism yet increases mortality.
    Authors: Shalhoub V, Shatzen EM, Ward SC
    J. Clin. Invest., 2012;122(7):2543-53.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 205  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ELISA.

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 ELISA Human Anonymous 01/08/2018
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Application ELISA
Sample Tested Purified Standard
Species Human

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