Human FGF-6 Antibody Summary
Accession # P10767
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF238). The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).
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- Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
- de Lapeyriere, O. et al. (1993) Development 118:601.
- Li, J. et al. (1993) Cancer 72:2253.
- Floss, T. et al. (1997) Genes Dev. 11:2040.
- Grass, S. et al. (1996) Development 122:141.
Citations for Human FGF-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Embryonic myogenesis pathways in muscle regeneration.
Authors: Zhao P, Hoffman EP
Dev. Dyn., 2004;229(2):380-92.
Sample Types: Whole Tissue
FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
Authors: Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L
J. Cell. Physiol., 2004;201(3):409-19.
Sample Types: Cell Lysates
Applications: Western Blot
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