Human FGF-6 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FGF-6 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human FGF-6
    Gly41-Ile208
    Accession # P10767
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human FGF‑6 (Catalog # 238-F6)
  • Neutralization
    Measured by its ability to neutralize FGF‑6-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human FGF‑6 and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF‑6 (Catalog # 238-F6) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF238). The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-6
Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).
  • References:
    1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
    2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    3. Marics, I. et al. (1989) Oncogene 4:335.
    4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
    5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
    6. Asada, M. et al. (1999) Growth Factors 16:293.
    7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
    8. de Lapeyriere, O. et al. (1993) Development 118:601.
    9. Li, J. et al. (1993) Cancer 72:2253.
    10. Floss, T. et al. (1997) Genes Dev. 11:2040.
    11. Grass, S. et al. (1996) Development 122:141.
    12. Long Name:
      Fibroblast Growth Factor 6
    13. Entrez Gene IDs:
      2251 (Human); 14177 (Mouse); 170700 (Rat)
    14. Alternate Names:
      FGF6; FGF-6; fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST2; HST-2; HSTF2; HSTF-2
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    2 Citations: Showing 1 - 2
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    Species
    Applications
    Sample Type
    1. FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
      Authors: Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L
      J. Cell. Physiol., 2004;201(3):409-19.
      Species: Mouse
      Sample Type: Cell Lysates
      Application: WB
    2. Embryonic myogenesis pathways in muscle regeneration.
      Authors: Zhao P, Hoffman EP
      Dev. Dyn., 2004;229(2):380-92.
      Species: Mouse
      Sample Type: Whole Tissue
      Application: IHC
    Isotype Controls
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    Ctrl AB-108-C 191  
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    Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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