Human FGF-6 Antibody

Catalog # Availability Size / Price Qty
Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody.
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Product Details
Citations (2)
Supplemental Products

Human FGF-6 Antibody Summary

Species Reactivity
Detects human FGF-6 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta -ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human FGF-6
Accession # P10767
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human FGF‑6 (Catalog # 238-F6)
Measured by its ability to neutralize FGF‑6-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Human FGF‑6 and 1 µg/mL heparin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. View Larger

Cell Proliferation Induced by FGF‑6 and Neutralization by Human FGF‑6 Antibody. Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF238). The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-6

Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Mature human FGF-6 is a 171 aa protein that shares 94% aa sequence identity with mouse and rat FGF-6 (3). It binds and signals primarily through FGF R1c, 2c, and 4 (4). FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect (5‑7). FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma (7-9). In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration (10). FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites (8, 11).

  1. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  3. Marics, I. et al. (1989) Oncogene 4:335.
  4. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  5. Pizette, S. et al. (1991) Cell Growth Differ. 2:561.
  6. Asada, M. et al. (1999) Growth Factors 16:293.
  7. Ropiquet, F. et al. (2000) Cancer Res. 60:4245.
  8. de Lapeyriere, O. et al. (1993) Development 118:601.
  9. Li, J. et al. (1993) Cancer 72:2253.
  10. Floss, T. et al. (1997) Genes Dev. 11:2040.
  11. Grass, S. et al. (1996) Development 122:141.
    Long Name
    Fibroblast Growth Factor 6
    Entrez Gene IDs
    2251 (Human); 14177 (Mouse); 170700 (Rat)
    Alternate Names
    FGF6; FGF-6; fibroblast growth factor 6; HBGF-6; Heparin secretory-transforming protein 2; Heparin-binding growth factor 6; HST2; HST-2; HSTF2; HSTF-2

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    Citations for Human FGF-6 Antibody

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    2 Citations: Showing 1 - 2
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    1. Embryonic myogenesis pathways in muscle regeneration.
      Authors: Zhao P, Hoffman EP
      Dev. Dyn., 2004;229(2):380-92.
      Species: Mouse
      Sample Types: Whole Tissue
      Applications: IHC
    2. FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
      Authors: Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L
      J. Cell. Physiol., 2004;201(3):409-19.
      Species: Mouse
      Sample Types: Cell Lysates
      Applications: Western Blot


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    Isotype Controls

    Reconstitution Buffers

    Secondary Antibodies

    Goat IgG (H+L) Antibody


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