Human FGF acidic/FGF1 Antibody Summary
Phe16-Asp155
Accession # P05230
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human FGF acidic/FGF1 by Western Blot. Western blot shows lysates of human brain (hypothalamas) tissue and human heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF acidic/FGF1 at approximately 16-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FGF acidic/FGF1 in Human Breast. FGF acidic/FGF1 was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Cell Proliferation Induced by FGF acidic/FGF1 and Neutralization by Human FGF acidic/FGF1 Antibody. Recombinant Human FGF acidic/FGF1 aa 16-155 (Catalog # 232-FA) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF acidic/FGF1 aa 16-155 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232). The ND50 is typically < 2 µg/mL in the presence of heparin (10 µg/mL).
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Detection of Human FGF acidic/FGF1 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue, loaded at 0.2 mg/mL. A specific band was detected for FGF acidic/FGF1 at approximately 25 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human FGF acidic/FGF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF232) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF acidic/FGF1
FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%‑33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).
- Jaye, M. et al. (1986) Science 233:541.
- Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
- Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
- Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073.
- Rajalingam, D. et al. (2007) Biochemistry 46:9225.
- Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
- Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
- Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
- Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
- Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
- Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
- Imamura, T. et al. (1990) Science 249:1567.
- Wesche, J. et al. (2005) Biochemistry 44:6071.
- Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
- Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
- Bouleau, S. et al. (2005) Oncogene 24:7839.
Product Datasheets
Citations for Human FGF acidic/FGF1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Tumor-derived Immunoglobulin-like transcript 4 facilitates angiogenesis of colorectal cancer
Authors: J Liu, F Zhang, J He, S Wang, L Wang, J Li, W Shi, Y Han, A Gao, Y Sun
American journal of cancer research, 2023-02-15;13(2):419-435.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer
Authors: HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith
British Journal of Cancer, 2022-07-01;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells
Authors: A Lampart, KD Sluzalska, A Czyrek, A Szerszen, J Otlewski, A Wiedlocha, M Zakrzewska
Cells, 2022-02-02;11(3):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Pituitary Adenylate Cyclase-Activating Peptide (PACAP), a Novel Secretagogue, Regulates Secreted Morphogens in Newborn Rat Retina
Authors: M Lakk, V Denes, K Kovacs, O Hideg, BF Szabo, R Gabriel
Invest. Ophthalmol. Vis. Sci, 2017-01-01;58(1):565-572.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Beneficial effects of coculturing synovial derived mesenchymal stem cells with meniscus fibrochondrocytes are mediated by fibroblast growth factor 1: increased proliferation and collagen synthesis.
Authors: Song, Xuanhe, Xie, Yaoping, Liu, Yang, Shao, Ming, Wang, Wenbo
Stem Cells Int, 2015-03-16;2015(0):926325.
Species: Human
Sample Types: Whole Cells
Applications: IHC-P -
WNT7A/beta-catenin signaling induces FGF1 and influences sensitivity to niclosamide in ovarian cancer.
Authors: King M, Lindberg M, Stodden G, Okuda H, Ebers S, Johnson A, Montag A, Lengyel E, MacLean Ii J, Hayashi K
Oncogene, 2014-09-01;34(26):3452-62.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Reduction of brain metastases in plasminogen activator inhibitor-1-deficient mice with transgenic ocular tumors.
Authors: Maillard CM, Bouquet C, Petitjean MM, Mestdagt M, Frau E, Jost M, Masset AM, Opolon PH, Beermann F, Abitbol MM, Foidart JM, Perricaudet MJ, Noel AC
Carcinogenesis, 2008-08-27;29(11):2236-42.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Molecular profiling of cervical cancer progression.
Authors: Hagemann T, Bozanovic T, Hooper S, Ljubic A, Slettenaar VI, Wilson JL, Singh N, Gayther SA, Shepherd JH, Van Trappen PO
Br. J. Cancer, 2007-01-29;96(2):321-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
Development, 2005-02-16;132(6):1223-34.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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