Detects IIIb isoforms of human FGF R1. In direct ELISAs and Western blots, this antibody detected IIIb but not IIIc isoforms of human FGF R1, human FGF R2, and mouse FGF R2. In direct ELISA, approximately 20% cross-reactivity was observed with recombinant human (rh) FGF R3 (IIIb). No cross-reactivity was observed with rhFGF R4 or with IIIIc isoforms of rhFGF R1, rhFGF R2, rmFGF R2, or rhFGF R3.
Monoclonal Mouse IgG1 Clone # 133111
Protein A or G purified from hybridoma culture supernatant
A mixture of purified isoforms of recombinant human FGF R1 extracellular domains
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR)
Measured by its ability to neutralize FGF R1 alpha -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 6 ng/mL Recombinant Human FGF R1 alpha (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
FGF R1 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody. Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1 alpha (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND50 is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF R1
FGF R1 occurs as multiple isoforms. The extracellular domain consists
of three ( alpha isoforms) or two ( beta isoforms) immunoglobulin-like domains.
In addition, the membrane-proximal Ig-like domain can be encoded by
alternative exons yielding (IIIb) or (IIIc) isoforms.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.