Human FGFR1 (IIIb) Antibody

FGF R1 alpha Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R1 Antibody.

Recombinant Human FGF R1a (IIIb) Fc Chimera (Catalog # 655-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R1a (IIIb) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R1 (IIIb) Monoclonal Antibody (Catalog # MAB765). The ND₅₀ is typically 0.075-0.3 µg/mL in the presence of heparin (10 µg/mL).

Detection of Human FGFR1 by Immunocytochemistry/Immunofluorescence

Proximity between AcSDKP and FGFR1 inhibits the TGF beta /smad signaling pathway in HMVECs. (a) HMVECs were treated with N-FGFR1 (1.5  μg/ml) for 48 h with or without preincubation with AcSDKP (100 nM) for 2 h, and the proximity between AcSDKP and FGFR1 was analyzed by the Duolink In Situ Assay. For each slide, images at a × 400 original magnification were obtained from six different areas. (b and c) HMVECs were treated with TGF beta 2 (5 ng/ml) for 15 min or 48 h with or without preincubation with AcSDKP for 2 h, and the p-smad3, TGF beta R1, TGF beta R2 and FGFR1 levels were analyzed by western blot. Densitometric analysis of the p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin levels from each group (n=6) were analyzed. (d and e) HMVECs were incubated with TGF beta 2 for 15 min or 48 h with or without preincubation with AcSDKP or its mutants (AcDSPK, AcSDKA, AcADKP) (100 nM) for 2 h. The p-smad3/smad3, TGF beta R1/ beta -actin, TGF beta R2/ beta -actin and FGFR1/ beta -actin protein levels were analyzed by western blot Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28771231), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human FGFR1 by Western Blot

Expression of FGFR1 in cells from breast and bone tissue. a, FGFR mRNA transcript levels in normal and cancer cells. b, Fold change in FGFR mRNA based on expression in cancers cells relative to normal cells. c, FGFR protein levels in normal versus cancer cells. Results are from triplicate experiments and the Western blot is representative of the triplicates. (*p < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26201468), licensed under a CC-BY license. Not internally tested by R&D Systems.