Detects the IIIb and IIIc isoforms of human FGF R3. It does not cross-react with mouse FGF R3 or with any isoforms of recombinant human (rh) FGF R1 or rhFGF R2.
Monoclonal Mouse IgG1 Clone # 136318
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human FGF R3 alpha (IIIc)
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR)
Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. Raines, E.W. et al. (1985) Methods Enzymol. 109: 749.The Neutralization Dose (ND50) is typically 2.5-15 ng/mL in the presence of 6 ng/mL Recombinant Human FGF R3 (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R3 Antibody. Recombinant Human FGF R3 (IIIc) Fc Chimera (Catalog # 766-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIc) Fc Chimera (6 ng/mL) is neutralized (green line) by increasing concentrations of Human FGF R3 Monoclonal Antibody (Catalog # MAB7661). The ND50 is typically 2.5-15 ng/mL in the presence of heparin (10 µg/mL).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF R3
The extracellular domain of FGF R3 includes 3 Ig-like domains. A
segment of the membrane proximal Ig-like domain can be encoded by two
different exons resulting in (IIIb) or (IIIc) isoforms.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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