Human Fibronectin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Fibronectin in A549 Human Cell Line. Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Fibronectin in Human Liver. Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibronectin
Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.
Product Datasheets
Citations for Human Fibronectin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastoma
Authors: T van Gronin, N Akogul, EM Westerhout, A Chan, NE Hasselt, DA Zwijnenbur, M Broekmans, P Stroeken, F Haneveld, GKJ Hooijer, CD Savci-Heij, A Lakeman, R Volckmann, P van Sluis, LJ Valentijn, J Koster, R Versteeg, J van Nes
Nat Commun, 2019;10(1):1530.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes
Authors: AL Jolly, S Rau, AK Chadha, EA Abdulrahee, D Dean
MBio, 2019;10(2):.
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF -
Presence of Stromal Cells Enhances Epithelial-to-Mesenchymal Transition (EMT) Induction in Lung Bronchial Epithelium after Protracted Exposure to Oxidative Stress of Gamma Radiation
Authors: A Acheva, S Haghdoost, A Sollazzo, V Launonen, M Kämäräinen
Oxid Med Cell Longev, 2019;2019(0):4120379.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neuroblastoma is composed of two super-enhancer-associated differentiation states
Authors: T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg
Nat. Genet., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
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PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
J. Cell. Sci., 2012;125(0):2276-87.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Semen clusterin is a novel DC-SIGN ligand.
Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, RodrÃgues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
J. Immunol., 2011;187(10):5299-309.
Species: Human
Sample Types: Seminal Plasma
Applications: Western Blot -
Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
Authors: Massam-Wu T, Chiu M, Choudhury R
J. Cell. Sci., 2010;123(0):3006-18.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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Paired with MAB1918