Fibronectin in A549 Human Cell Line.
Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Fibronectin in Human Liver.
Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.
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Neuroblastoma is composed of two super-enhancer-associated differentiation states
Authors: T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg
Nat. Genet., 2017;0(0):.
Sample Type: Cell Lysates
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We have 1 review tested in 1 application: ELISA.