Human Fibronectin Antibody

Name: Human Fibronectin Antibody
Brand: R&D Systems
Rating: 4 (1 reviews)

AF1918 4 Citations 1 Review Datasheet / COA / SDS

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AF1918
AF1918-SP

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Fibronectin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Fibronectin in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Human plasma-derived Fibronectin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Human Fibronectin (Catalog # 1918-FN)

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry

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Fibronectin in A549 Human Cell Line. Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Fibronectin in Human Liver. Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibronectin

Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Alternate Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Fibronectin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Neuroblastoma is composed of two super-enhancer-associated differentiation states
Authors: T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg
Nat. Genet., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
J. Cell. Sci., 2012;125(0):2276-87.
Species: Human
Sample Types: Tissue Homogenates
Applications: WB
Semen clusterin is a novel DC-SIGN ligand.
Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, RodrÃgues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
J. Immunol., 2011;187(10):5299-309.
Species: Human
Sample Types: Seminal Plasma
Applications: WB
Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
Authors: Massam-Wu T, Chiu M, Choudhury R
J. Cell. Sci., 2010;123(0):3006-18.
Species: Human
Sample Types: Whole Cells
Applications: ICC