Human Fibronectin Antibody
Human Fibronectin Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Fibronectin in A549 Human Cell Line. Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Fibronectin in Human Liver. Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fibronectin
Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.
Product Datasheets
Citations for Human Fibronectin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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A NOTCH feed-forward loop drives reprogramming from adrenergic to mesenchymal state in neuroblastoma
Authors: T van Gronin, N Akogul, EM Westerhout, A Chan, NE Hasselt, DA Zwijnenbur, M Broekmans, P Stroeken, F Haneveld, GKJ Hooijer, CD Savci-Heij, A Lakeman, R Volckmann, P van Sluis, LJ Valentijn, J Koster, R Versteeg, J van Nes
Nat Commun, 2019;10(1):1530.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Stromal Fibroblasts Drive Host Inflammatory Responses That Are Dependent on Chlamydia trachomatis Strain Type and Likely Influence Disease Outcomes
Authors: AL Jolly, S Rau, AK Chadha, EA Abdulrahee, D Dean
MBio, 2019;10(2):.
Species: Human
Sample Types: Whole Cells
Applications: ICC/IF -
Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.
Authors: Sandeep Kumar Vishwakarm, Avinash Bardia, Chandrakala Lakkiredd, Syed Ameer Basha Paspala, Aleem Ahmed Khan
Frontiers in Bioengineering and Biotechnology, 2018;0(0):2296-4185.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Neuroblastoma is composed of two super-enhancer-associated differentiation states
Authors: T van Gronin, J Koster, LJ Valentijn, DA Zwijnenbur, N Akogul, NE Hasselt, M Broekmans, F Haneveld, NE Nowakowska, J Bras, CJM van Noesel, A Jongejan, AH van Kampen, L Koster, F Baas, L van Dijk-K, M Huizer-Smi, MC Lecca, A Chan, A Lakeman, P Molenaar, R Volckmann, EM Westerhout, M Hamdi, PG van Sluis, ME Ebus, JJ Molenaar, GA Tytgat, BA Westerman, J van Nes, R Versteeg
Nat. Genet., 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
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PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation.
J. Cell. Sci., 2012;125(0):2276-87.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Semen clusterin is a novel DC-SIGN ligand.
Authors: Sabatte J, Faigle W, Ceballos A, Morelle W, RodrÃgues CR, Lenicov FR, Thepaut M, Fieschi F, Malchiodi E, Fernandez M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
J. Immunol., 2011;187(10):5299-309.
Species: Human
Sample Types: Seminal Plasma
Applications: Western Blot -
Assembly of fibrillin microfibrils governs extracellular deposition of latent TGF beta.
Authors: Massam-Wu T, Chiu M, Choudhury R
J. Cell. Sci., 2010;123(0):3006-18.
Species: Human
Sample Types: Whole Cells
Applications: ICC
FAQs
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Secondary Antibodies
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