Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat, Ovine

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Fibronectin Primary Antibodies »

Product Specifications

Immunogen

Human plasma-derived Fibronectin

Specificity

Detects human Fibronectin in direct ELISAs. Detects human and rat Fibronectin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Rat Fibronectin Antibody

Detection of Human Fibronectin by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Fibronectin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Fibronectin antibody in A549 Human Cell Line by Immunocytochemistry (ICC).

Fibronectin in A549 Human Cell Line.

Fibronectin was detected in immersion fixed A549 human lung carcinoma cell line untreated (top panel) or treated with 10 ng/mL TGF-beta (Catalog # 240-B, lower panel) for 48 hours using Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Fibronectin antibody in Human Liver by Immunohistochemistry (IHC-P).

Fibronectin in Human Liver.

Fibronectin was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human Fibronectin by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Fibronectin at approximately 230 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Rat Fibronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1918) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016).The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple WesternTM

Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

B7‐H3 interacts with FN. (A) TCGA database analysis of B7‐H3 and the correlation coefficient of FN’s two genes. (B, C), The interaction of B7‐H3 with FN in 786‐O and ACHN cells detected by co‐IP assay. (D, E) The expression of FN in the B7‐H3‐KO and control 786‐O and ACHN cells assayed by western blotting. (F) The expression of FN in the B7‐H3‐KO and control ACHN cells isolated from immunodeficient mice as assayed by immunohistochemical staining. After 6 weeks of injection, tumor tissues were collected and immunohistochemical methods were used to detect FN expression levels in tumor tissues. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01 B7‐H3, control group vs. B7‐H3‐KO group. Scale bars: 25 μm (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Immunohistochemistry

Detection of Fibronectin by Immunohistochemistry

B7‐H3 interacts with FN. (A) TCGA database analysis of B7‐H3 and the correlation coefficient of FN’s two genes. (B, C), The interaction of B7‐H3 with FN in 786‐O and ACHN cells detected by co‐IP assay. (D, E) The expression of FN in the B7‐H3‐KO and control 786‐O and ACHN cells assayed by western blotting. (F) The expression of FN in the B7‐H3‐KO and control ACHN cells isolated from immunodeficient mice as assayed by immunohistochemical staining. After 6 weeks of injection, tumor tissues were collected and immunohistochemical methods were used to detect FN expression levels in tumor tissues. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01 B7‐H3, control group vs. B7‐H3‐KO group. Scale bars: 25 μm (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

Activation of PI3K/AKT and p38/ERK MAPK signaling by FN. (A, B) The expression of EMT‐related proteins in FN‐treated control and KO cells. (C, D) The expression of signaling pathway‐related proteins in FN‐treated control and KO cells. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, B7‐H3 control vs. FN‐treated control and KO cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

B7‐H3 interacts with FN. (A) TCGA database analysis of B7‐H3 and the correlation coefficient of FN’s two genes. (B, C), The interaction of B7‐H3 with FN in 786‐O and ACHN cells detected by co‐IP assay. (D, E) The expression of FN in the B7‐H3‐KO and control 786‐O and ACHN cells assayed by western blotting. (F) The expression of FN in the B7‐H3‐KO and control ACHN cells isolated from immunodeficient mice as assayed by immunohistochemical staining. After 6 weeks of injection, tumor tissues were collected and immunohistochemical methods were used to detect FN expression levels in tumor tissues. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01 B7‐H3, control group vs. B7‐H3‐KO group. Scale bars: 25 μm (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

Activation of PI3K/AKT and p38/ERK MAPK signaling by FN. (A, B) The expression of EMT‐related proteins in FN‐treated control and KO cells. (C, D) The expression of signaling pathway‐related proteins in FN‐treated control and KO cells. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, B7‐H3 control vs. FN‐treated control and KO cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

FN binds to B7‐H3 and promotes cell migration and invasion. (A, B) Migration and invasion ability of si‐NC and si‐FN groups examined by transwell assay. (C, D) The binding level of exogenous FN to the control and B7‐H3‐KO cells. (E, F) Migration and invasion ability of the control and B7‐H3‐KO cells that were cultured with exogenous FN. Values are expressed as means ± SD (t‐tests, n = 3). Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, FN control group vs. si‐FN group (A, B); *P < 0.05, **P < 0.01, ***P < 0.001, B7‐H3 control vs. FN‐treated control and KO cells. Scale bars: 25 μm (A, B, E, F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

Activation of PI3K/AKT and p38/ERK MAPK signaling by FN. (A, B) The expression of EMT‐related proteins in FN‐treated control and KO cells. (C, D) The expression of signaling pathway‐related proteins in FN‐treated control and KO cells. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, B7‐H3 control vs. FN‐treated control and KO cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Fibronectin by Western Blot

Detection of Fibronectin by Western Blot

B7‐H3 interacts with FN. (A) TCGA database analysis of B7‐H3 and the correlation coefficient of FN’s two genes. (B, C), The interaction of B7‐H3 with FN in 786‐O and ACHN cells detected by co‐IP assay. (D, E) The expression of FN in the B7‐H3‐KO and control 786‐O and ACHN cells assayed by western blotting. (F) The expression of FN in the B7‐H3‐KO and control ACHN cells isolated from immunodeficient mice as assayed by immunohistochemical staining. After 6 weeks of injection, tumor tissues were collected and immunohistochemical methods were used to detect FN expression levels in tumor tissues. Values are expressed as means ± SD (t‐tests, n = 3). *P < 0.05, **P < 0.01 B7‐H3, control group vs. B7‐H3‐KO group. Scale bars: 25 μm (F). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34431237), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Rat Fibronectin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line untreated or treated with 10 ng/ML TGF-beta (Catalog # 240-B) for 48 hours

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

10 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and C6 rat glioma cell line

Reviewed Applications

Read 3 reviews rated 4 using AF1918 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Fibronectin

Plasma Fibronectin is a secreted dimeric protein. It is involved in cell adhesion, migration, wound healing, and other cellular processes. Human Fibronectin shares 92% and 90% amino acid sequence homology with bovine and mouse Fibronectin, respectively.

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Human/Rat Fibronectin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Rat Fibronectin Antibody

For research use only

Citations for Human/Rat Fibronectin Antibody

Customer Reviews for Human/Rat Fibronectin Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Rat Fibronectin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Human Fibronectin Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 10/01/2019
    Paired with MAB1918
    Human/Rat Fibronectin Antibody AF1918
  • Human Fibronectin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 20699357
    Species: Human
    Verified Customer | Posted 01/05/2015

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies