Human Follistatin-related Gene Protein/FLRG Antibody Summary
Accession # O95633
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemo-globin Expression and Neutralization by Human Follistatin-related Gene Protein/FLRG Antibody. Recombinant Human Follistatin-related Gene Protein/FLRG (Catalog # 1288-F3) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin-related Gene Protein/ FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1288). The ND50 is typically 1-4 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin-related Gene Protein/FLRG
Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).
- Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
- Sidis, Y. et al. (2002) Endocrinology 143:1613.
- Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
- Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
- Bartholin, L. et al. (2001) Oncogene 20:5409.
Citation for Human Follistatin-related Gene Protein/FLRG Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks.
Authors: Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH
Prenat. Diagn., 2011;31(2):135-41.
Sample Types: Serum
Applications: ELISA Development
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