Human Follistatin-related Gene Protein/FLRG Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects Follistatin-related Gene Protein/FLRG in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse FLRG is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FLRG
    Met27-Val263
    Accession # O95633
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Follistatin-related Gene Protein/FLRG (Catalog # 1288-F3)
  • Neutralization
    Measured by its ability to neutralize Follistatin‑related Gene Protein/FLRG inhibition of Activin A-dependent hemoglobin expression in the K562 human chronic myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 0.1 µg/mL Recombinant Human Follistatin‑related Gene Protein/FLRG and 7.5 ng/mL Recombinant Human/Mouse/Rat Activin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Follistatin-related Gene Protein/FLRG Inhibition of Activin A-induced Hemo­globin Expression and Neutralization by Human Follistatin-related Gene Protein/FLRG Antibody. Recombinant Human Follistatin-related Gene Protein/FLRG (Catalog # 1288-F3) inhibits Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induced hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase activity. Inhibition of Recombinant Human/Mouse/Rat Activin A (7.5 ng/mL) activity elicited by Recombinant Human Follistatin‑related Gene Protein/
FLRG (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Follistatin-related Gene Protein/FLRG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1288). The ND50 is typically 1‑4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Follistatin-related Gene Protein/FLRG

Follistatin-related gene protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Human FLRG cDNA encodes a 263 amino acid (aa) residue protein with a putative 26 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta  and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1‑5).

  • References:
    1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
    2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
    3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
    4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
    5. Bartholin, L. et al. (2001) Oncogene 20:5409.
  • Entrez Gene IDs:
    10272 (Human); 83554 (Mouse)
  • Alternate Names:
    FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Prediction of gestational diabetes mellitus by maternal factors and biomarkers at 11 to 13 weeks.
    Authors: Nanda S, Savvidou M, Syngelaki A, Akolekar R, Nicolaides KH
    Prenat. Diagn., 2011;31(2):135-41.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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