Human FRS2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FRS2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 462902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FRS2
    Asn121-Asn449
    Accession # Q8WU20
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of FRS2 in A172 Human Cell Line by Flow Cytometry.
A172 human glioblastoma cell line was stained with Mouse Anti-Human FRS2 Monoclonal Antibody (Catalog # MAB40691, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FRS2

FRS2 (FGFR substrate 2; also SNT and FSR2-alpha ) is a 70‑90 kDa member of the FSR family of lipid-anchored docking proteins. It is an intermediary between FGF and TRK receptors, and their Ras/MAPK signaling cascades. Human FRS2 is 512 amino acids (aa) in length. It contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain that interacts with FGF and NGF receptors (aa 13‑115), and a C-terminal Tyr-rich region that serves as a docking site for Grb2 and Shp2 (aa 196‑471). One splice form shows a deletion of the C-terminal four amino acids. Over aa 121‑449, human FRS2 is 99% and 94% aa identical to dog and mouse FRS2, respectively.

  • Long Name:
    Fibroblast Growth Factor Receptor Substrate 2
  • Entrez Gene IDs:
    10818 (Human); 327826 (Mouse)
  • Alternate Names:
    FGFR signalling adaptor; FGFR-signaling adaptor SNT; fibroblast growth factor receptor substrate 2; FRS2; FRS2A; FRS2alpha; SNT; SNT1; SNT-1FGFR substrate 2; Suc1-associated neurotrophic factor target 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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