Human gamma -Synuclein Antibody MAB5745: R&D Systems

Human gamma -Synuclein Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human gamma -Synuclein in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 514304
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human gamma -Synuclein
    Asn47-Glu120
    Accession # Q6FHG5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human brain (cortex and hippocampus)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human gamma -Synuclein by Western Blot. Western blot shows lysates of human hypothalamus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for gamma -Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: gamma-Synuclein

gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

  • Entrez Gene IDs:
    6623 (Human)
  • Alternate Names:
    BCSG1; BCSG1PERSYN; Breast cancer-specific gene 1 protein; gamma-Synuclein; persyn; PRSN; SNCG; SRPRSN; Synoretin; synuclein, gamma (breast cancer-specific protein 1); Synuclein-gamma
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