Human gamma-Synuclein Antibody

Catalog #: MAB5745
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5745
MAB5745-SP

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Detection of Human gamma -Synuclein by Western Blot.

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Citations (2)

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Human gamma-Synuclein Antibody Summary

Species Reactivity

Human

Specificity

Detects human gamma -Synuclein in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 514304

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human gamma -Synuclein
Asn47-Glu120
Accession # Q6FHG5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human brain (cortex and hippocampus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human gamma -Synuclein by Western Blot. Western blot shows lysates of human hypothalamus tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for gamma-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Synuclein

gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Entrez Gene IDs

6623 (Human)

Alternate Names

BCSG1; BCSG1PERSYN; Breast cancer-specific gene 1 protein; gamma-Synuclein; persyn; PRSN; SNCG; SRPRSN; Synoretin; synuclein, gamma (breast cancer-specific protein 1); Synuclein-gamma

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human gamma-Synuclein Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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