Human gamma -Synuclein Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB5745R-SP
MAB5745R-500
MAB5745R-01M
MAB5745R-100
Detection  of Human  gamma -Synuclein by Western Blot.
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Human gamma -Synuclein Antibody Summary

Species Reactivity
Human
Specificity
Detects human gamma -Synuclein in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 514304R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human gamma -Synuclein
Asn47-Glu120
Accession # Q6FHG5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human  gamma -Synuclein by Western Blot. View Larger

Detection of Human gamma -Synuclein by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Recombinant Mouse Anti-Human
gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gamma -Synuclein at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry gamma -Synuclein in MBA‑MB‑468 Human Cell Line. View Larger

gamma -Synuclein in MBA‑MB‑468 Human Cell Line. gamma -Synuclein was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Recombinant Mouse Anti-Human gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry   gamma -Synuclein  in Human Brain. View Larger

  gamma -Synuclein in Human Brain. gamma -Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Recombinant Mouse Anti-Human gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Synuclein

gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Entrez Gene IDs
6623 (Human)
Alternate Names
BCSG1; BCSG1PERSYN; Breast cancer-specific gene 1 protein; gamma-Synuclein; persyn; PRSN; SNCG; SRPRSN; Synoretin; synuclein, gamma (breast cancer-specific protein 1); Synuclein-gamma

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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