Human gamma -Synuclein Antibody Summary
Accession # Q6FHG5
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human gamma -Synuclein by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Recombinant Mouse Anti-Human
gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gamma -Synuclein at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
gamma -Synuclein in MBA‑MB‑468 Human Cell Line. gamma -Synuclein was detected in immersion fixed MBA‑MB‑468 human breast cancer cell line using Recombinant Mouse Anti-Human gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
gamma -Synuclein in Human Brain. gamma -Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Recombinant Mouse Anti-Human gamma -Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
gamma -Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Cell and Tissue Staining Kits
Reviews for Human gamma -Synuclein Antibody
There are currently no reviews for this product. Be the first to review Human gamma -Synuclein Antibody and earn rewards!
Have you used Human gamma -Synuclein Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image