Human Gas1 Antibody
Human Gas1 Antibody Summary
Accession # P54826
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The human Gas1 cDNA encodes a 345 amino acid (aa) precursor that includes a 39 aa signal sequence, a 279 aa mature protein, and a 27 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature human and mouse Gas1 share 85% aa sequence identity. Human Gas1 is a 40 kDa GPI-linked glycoprotein that is uniformly distributed on the cell surface (7). In contact-inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental actions including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10‑14). Gas1 contributes to the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 function on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems data which demonstrate direct binding of Gas1 to Artemin and Neurturin.
- Schneider, C. et al. (1988) Cell 54:787.
- Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
- Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
- Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
- Del Sal, G. et al. (1992) Cell 70:595.
- Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
- Stebel, M. et al. (2000) FEBS Lett. 481:152.
- Spagnuolo, R. et al. (2004) Blood 103:3005.
- Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
- Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
- Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
- Liu, Y. et al. (2002) Development 129:5289.
- Liu, Y. et al. (2001) Dev. Biol. 236:30.
- Lee, C.S. et al. (2001) Dev. Biol. 236:17.
- Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.
Citation for Human Gas1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration
Authors: Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim
Nat Commun, 2020;11(1):1994.
Sample Types: Whole Tissue
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Gas1 Antibody
There are currently no reviews for this product. Be the first to review Human Gas1 Antibody and earn rewards!
Have you used Human Gas1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image