Human GCNF/NR6A1 Antibody Summary
Accession # Q15406
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
GCNF/NR6A1 in HepG2 Human Cell Line. GCNF/NR6A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and MOLT‑4 human acute lymphoblastic leukemia cell line (negative staining) using Goat Anti-Human GCNF/NR6A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3180) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GCNF, also known as retinoid receptor-related testis-associated receptor (RTR), is a member of the nuclear receptor superfamily and has been designated NR6A1. It plays important roles in embryogenesis and cell differentiation. Over the sequence used for immunization, human and mouse GCNF share 98.5% amino acid sequence identity.
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