Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GCNF/NR6A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GCNF/NR6A1
Met215-Glu480
Accession # Q15406

Specificity

Detects human and mouse GCNF/NR6A1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human GCNF/NR6A1 Antibody

GCNF/NR6A1 in HepG2 Human Cell Line.

GCNF/NR6A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and MOLT‑4 human acute lymphoblastic leukemia cell line (negative staining) using Goat Anti-Human GCNF/NR6A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3180) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GCNF/NR6A1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human GCNF/NR6A1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GCNF/NR6A1

GCNF, also known as retinoid receptor-related testis-associated receptor (RTR), is a member of the nuclear receptor superfamily and has been designated NR6A1. It plays important roles in embryogenesis and cell differentiation. Over the sequence used for immunization, human and mouse GCNF share 98.5% amino acid sequence identity.

Long Name

Germ Cell Nuclear Factor

Alternate Names

NR6A1, RTR

Entrez Gene IDs

2649 (Human)

Gene Symbol

NR6A1

UniProt

Q15406

Additional GCNF/NR6A1 Products

Product Documents for Human GCNF/NR6A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GCNF/NR6A1 Antibody

For research use only

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Protocols

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