Human GCNF/NR6A1 Antibody

Catalog #: AF3180 Datasheet / COA / SDS

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AF3180
AF3180-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GCNF/NR6A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human and mouse GCNF/NR6A1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GCNF/NR6A1
Met215-Glu480
Accession # Q15406

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GCNF/NR6A1

Immunocytochemistry

5-15 µg/mL

Immersion fixed HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GCNF/NR6A1 in HepG2 Human Cell Line. GCNF/NR6A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line (positive staining) and MOLT‑4 human acute lymphoblastic leukemia cell line (negative staining) using Goat Anti-Human GCNF/NR6A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3180) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GCNF/NR6A1

GCNF, also known as retinoid receptor-related testis-associated receptor (RTR), is a member of the nuclear receptor superfamily and has been designated NR6A1. It plays important roles in embryogenesis and cell differentiation. Over the sequence used for immunization, human and mouse GCNF share 98.5% amino acid sequence identity.

Long Name

Germ Cell Nuclear Factor

Entrez Gene IDs

2649 (Human)

Alternate Names

GCNF; GCNF1; GCNFNR61; Germ cell nuclear factor; hGCNF; NR6A1; nuclear receptor subfamily 6 group A member 1; nuclear receptor subfamily 6, group A, member 1; retinoic acid receptor-related testis-associated receptor; Retinoid receptor-related testis-specific receptor; RTR; RTRhRTR