Human GFR alpha-1/GDNF R alpha-1 Antibody
Human GFR alpha-1/GDNF R alpha-1 Antibody Summary
Met1-Lys429
Accession # NP_665736
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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GFR alpha ‑1/GDNF R alpha ‑1 in Human Spinal Cord. GFRa-1/GDNF Ra-1 was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to ventral horn motoneurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.
Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
- Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
- Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
- Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Product Datasheets
Citations for Human GFR alpha-1/GDNF R alpha-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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APE1 Promotes Pancreatic Cancer Proliferation through GFRalpha1/Src/ERK Axis-Cascade Signaling in Response to GDNF
Authors: YD Choi, JY Jung, M Baek, S Khan, PI Song, S Ryu, JY Koo, SC Chauhan, A Tsin, C Choi, WJ Kim, M Kim
Int J Mol Sci, 2020;21(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
Authors: RL Tang, LQ Fan
Asian J. Androl., 2019;21(3):309-318.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
An anti-GDNF Family Receptor Alpha 1(GFRA1) Antibody-Drug Conjugate for the Treatment of Hormone Receptor-Positive Breast Cancer
Authors: S Bhakta, LM Crocker, Y Chen, M Hazen, MM Schutten, D Li, C Kuijl, R Ohri, F Zhong, KA Poon, MAT Go, E Cheng, R Piskol, R Firestein, A Fourie-O'D, KR Kozak, H Raab, JA Hongo, D Sampath, MS Dennis, RH Scheller, P Polakis, JR Junutula
Mol. Cancer Ther., 2017;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
Development, 2007;134(18):3327-38.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons.
Authors: Girard BM, Young BA, Buttolph TR, Locknar SA, White SL, Parsons RL
Neuroscience, 2006;139(4):1329-41.
Species: Guinea Pig
Sample Types: Whole Cells
Applications: ICC
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