Human GFR alpha-2/GDNF R alpha-2 Antibody

Catalog # Availability Size / Price Qty
AF613
AF613-SP

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Product Details
Citations (1)
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Reviews (2)

Human GFR alpha-2/GDNF R alpha-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross‑reactivity with recombinant mouse GFR alpha -2 is observed and less than 5% cross-reactivity with recombinant human GFR alpha -1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑2/GDNF R alpha ‑2
Ser22-Ser441
Accession # O00451
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GFR alpha ‑2/GDNF R alpha ‑2 Fc Chimera (Catalog # 613-FR)
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human dorsal root ganglia
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 2 ng/mL of Recombinant Human GDNF (Catalog # 212-GD) to immobilized Recombinant Human GFR alpha -2 Fc Chimera (Catalog # 613-FR) coated at 1 µg/mL (100 µL/well). At 20 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-2/GDNF R alpha-2

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of theTGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha -1 [GFR alpha -1], also known as Trn R1 or GFR alpha -2, also known as Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Human GFR alpha -2 cDNA encodes a 464 amino acid (aa) residue protein with a putative N-terminal 21 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a three aa residue (GPS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

References
  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 2
Entrez Gene IDs
2675 (Human); 14586 (Mouse)
Alternate Names
GDNF family receptor alpha 2; GDNF family receptor alpha-2; GDNF R alpha-2; GDNF receptor alpha-2; GDNF receptor beta; GDNFR-alpha-2; GDNFR-beta; GDNFRBRET ligand 2; GFR alpha2; GFR alpha-2; GFRA2; GFRa-2; GFR-alpha 2; GFR-alpha-2; glial cell line derived neurotrophic factor receptor, beta; Neurturin receptor alpha; NRTNR-alpha; NTNRA; NTNR-alpha; PI-linked cell-surface accessory protein; RETL2TGF-beta-related neurotrophic factor receptor 2; TGF-beta related neurotrophic factor receptor 2; TRN receptor, GPI-anchored; TRNR2NRTNR-ALPHA

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Citation for Human GFR alpha-2/GDNF R alpha-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons.
    Authors: Girard BM, Young BA, Buttolph TR, Locknar SA, White SL, Parsons RL
    Neuroscience, 2006-03-03;139(4):1329-41.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human GFR alpha-2/GDNF R alpha-2 Antibody

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Human GFR alpha-2/GDNF R alpha-2 Antibody
By Anonymous on 07/19/2021
Application: WB Sample Tested: fibroblasts Species: Human

Human GFR alpha-2/GDNF R alpha-2 Antibody
By Anonymous on 04/28/2021
Application: WB Sample Tested: Brain (hippocampus) Species: Human