Human GLP-1R Antibody

Catalog #: MAB2814
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2814
MAB2814-SP

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Detection of GLP‑1R in HEK293 Human Cell Line Transfected with Human GLP-1R and eGFP by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GLP-1R Antibody Summary

Species Reactivity

Human

Specificity

Stains human GLP-1R transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 197920

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GLP-1R
Arg24-Ser463
Accession # NP_002053

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GLP‑1R in HEK293 Human Cell Line Transfected with Human GLP-1R and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GLP-1R and eGFP was stained with and either (A) Mouse Anti-Human GLP-1R Monoclonal Antibody (Catalog # MAB2814) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1R

GLP-1R is a class BG protein-linked seven transmembrane domain protein that is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach. GLP-1R functions as a receptor for glucagon-like peptide 1 and regulates insulin and glucagon production.

Long Name

Glucagon-like Peptide 1 Receptor

Entrez Gene IDs

2740 (Human); 14652 (Mouse)

Alternate Names

GLP-1 receptor; GLP1R; GLP-1R; GLP-1-R; glucagon-like peptide 1 receptor; MGC138331

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citation for Human GLP-1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1