Human GLP-1R Antibody (197920)

R&D Systems | Catalog # MAB2814

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 197920
View all GLP-1R Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GLP-1R
Arg24-Ser463
Accession # NP_002053

Specificity

Stains human GLP-1R transfectants but not irrelevant transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GLP-1R Antibody (197920)

Detection of GLP-1R antibody in HEK293 Human Cell Line Transfected with Human GLP-1R and eGFP antibody by Flow Cytometry.

Detection of GLP‑1R in HEK293 Human Cell Line Transfected with Human GLP-1R and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human GLP-1R and eGFP was stained with and either (A) Mouse Anti-Human GLP-1R Monoclonal Antibody (Catalog # MAB2814) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human GLP-1R Antibody (197920)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human GLP-1R and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLP-1R

GLP-1R is a class BG protein-linked seven transmembrane domain protein that is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach. GLP-1R functions as a receptor for glucagon-like peptide 1 and regulates insulin and glucagon production.

Long Name

Glucagon-like Peptide 1 Receptor

Alternate Names

GLP1R

Entrez Gene IDs

2740 (Human); 14652 (Mouse)

Gene Symbol

GLP1R

UniProt

NP_002053

Additional GLP-1R Products

Product Documents for Human GLP-1R Antibody (197920)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GLP-1R Antibody (197920)

For research use only

Citations for Human GLP-1R Antibody (197920)

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