Human Glucuronosyltransferase 1A1/UGT1A1 Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glucuronosyltransferase 1A1/UGT1A1 in ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 856754
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Glucuronosyltransferase 1A1/UGT1A1
    Leu60-Thr168
    Accession # P22309
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human Glucuronosyltransferase 1A1/UGT1A1 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Glucuronosyltransferase 1A1/UGT1A1 in HepG2 Human Cell Line. Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Glucuronosyltransferase 1A1/UGT1A1 by Simple WesternTM. Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 59 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glucuronosyltransferase 1A1/UGT1A1

UGT1A1 (UDP-glucuronosyltransferase 1-A1; also UDPGT 1-1 and HUG-BR1) is a 52-57 kDa member of the UGT1A subfamily, UGT family of enzymes. UGT1A1 is expressed by the liver, and catalyzes the conjugation of glucuronic acid (GA) from UDPGA to lipophilic acceptors such as (anti-cancer) drugs and bilirubin. Addition of glucuronic acid increases target solubility and facilitates elimination. Mature human UGT1A1 is a 508 amino acid (aa) type I transmembrane ER glycoprotein. It contains a 465 aa luminal domain (aa 26-490) plus a 26 aa cytoplasmic region. The luminal domain is unusual in that aa 157-176 are embedded in the ER membrane. Amino acids 29-444 contain the enzyme active site. The cytoplasmic tail appears to mediate noncovalent homodimerization, and heterodimerization with UGT2B. The signal sequence (aa 1-25) is normally cleaved, but a Lys15Arg mutation blocks insertion into the ER membrane. There are multiple point mutations that impact enzyme activity. One potential splice form is reported that shows a six aa substitution for aa 289-533. Over aa 60-186, human UGT1A1 shares 64% aa identity with mouse UGT1A1.

  • Long Name:
    UDP Glucuronosyltransferase 1A1
  • Entrez Gene IDs:
    54658 (Human); 394436 (Mouse); 24861 (Rat)
  • Alternate Names:
    bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; Bilirubin-specific UDPGT isozyme 1; GNT1; GNT1EC 2.4.1.17; HUG-BR1; UDP glucuronosyltransferase 1 family, polypeptide A1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UDPGT 1-1; UDPGT; UG-BR1; UGT1; UGT1*1; UGT1.1; UGT1-01; UGT1A; UGT-1A; UGT1A1; UGT1AUDPGT 1-1; UGT1BILIQTL1
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Average Rating: 3 (Based on 1 review)

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Western Blot Human Glucuronosyltransferase 1A1/UGT1A1 Antibody MAB6490
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Western Blot Human Glucuronosyltransferase 1A1/UGT1A1 Antibody MAB6490
Western Blot: Human Glucuronosyltransferase 1A1/UGT1A1 Antibody [MAB6490]

Summary

ApplicationWestern Blot
Sample TestedLiver tissue

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