Human Glypican 5 Antibody
Human Glypican 5 Antibody Summary
Accession # P78333
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 5
The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that likely play a key role in embryonic morphogenesis (1‑4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N‑terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 5 (GPC-5) is synthesized as a 572 amino acid (aa) preproprecursor that contains a 24 aa signal sequence, a 532 aa mature region and a 16 aa C-terminal prosegment (6, 7). There are three potential N-linked, and five potential O-linked sites for glycosylation or glycanation. GPC-5 is believed to contain 6‑7 kDa of glycosylation and at least 55 kDa of proteoglycan. This is based on an assumption of the presence of one heparan sulfate chain of 36 kDa and one chrondroitin sulfate chain of 17 kDa (7, 8). When added to the core molecular weight of 59 kDa, the mature protein is approximately 120 kDa in size. To date, however, the actual size of native human GPC-5 has not been reported and the suggestion of a chrondroitin sulfate modification is based on the expression of human GPC-5 in COS-7 cells (7). Human to mouse, there is 88% aa identity over the mature region. Cells known to express GPC-5 are principally embryonic in nature, and include neurons and mesenchyme (1, 7). The function of GPC-5 is essentially unknown. As a glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (9, 10).
- Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.
- Filmus, J. (2001) Glycobiology 11:19R.
- De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.
- Filmus, J. and S.B. Selleck (2001) J. Clin. Invest.108:497.
- Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
- Veugelers, M. et al. (1997) Genomics 40:24.
- Saunders, S. et al. (1997) Dev. Biol. 19:78.
- Rapraeger, A. et al. (1985) J. Biol. Chem. 260:11046.
- Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
- Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
Citation for Human Glypican 5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation
Authors: GFA de Moraes, E Listik, GZ Justo, CM Vicente, L Toma
BMC molecular and cell biology, 2021;22(1):26.
Sample Types: Whole Cells
Applications: Flow Cytometry
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Glypican 5 Antibody
There are currently no reviews for this product. Be the first to review Human Glypican 5 Antibody and earn rewards!
Have you used Human Glypican 5 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image