Human GPR26 Antibody

Catalog # Availability Size / Price Qty
MAB8449
MAB8449-SP
Detection of GPR26 in HEK293 Human Cell Line Transfected with Human GPR26 and eGFP by Flow Cytometry.
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Human GPR26 Antibody Summary

Species Reactivity
Human
Specificity
Stains human GPR26 transfectants but not irrelevant transfectants in flow cytometry.
Source
Monoclonal Mouse IgG2B Clone # 891318
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPR26
Met1-Glu337
Accession # Q8NDV2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GPR26 antibody in HEK293 Human Cell Line Transfected with Human GPR26 and eGFP antibody by Flow Cytometry. View Larger

Detection of GPR26 in HEK293 Human Cell Line Transfected with Human GPR26 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GPR26 and eGFP was stained with either (A) Mouse Anti-Human GPR26 Monoclonal Antibody (Catalog # MAB8449) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR26

GPR26 is a brain-specific orphan G Protein-Coupled Receptor (GPCR) of the rhodopsin family. It is frequently reduced or absent in glioma cells and biopsies. Glioblastomas are highly malignant brain tumors. GPR26 has been proposed to be a genetic indicator of primary glioblastoma, suggesting that it could be a suppressor of primary glioblastoma development.

Long Name
G Protein-coupled Receptor 26
Entrez Gene IDs
2849 (Human); 233919 (Mouse)
Alternate Names
DKFZp761H2121; FLJ33774; G protein-coupled receptor 26; GPR26; G-protein coupled receptor 26; MGC138216

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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