Key Product Details

Species Reactivity

Validated:

Human

Cited:

Canine

Applications

Validated:

Flow Cytometry, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 481906
View all GPRC5A Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPRC5A
Met1-Ser357
Accession # Q8NFJ5

Specificity

Stains human GPRC5A transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human GPRC5A Antibody

Detection of GPRC5A antibody in A549 Human Cell Line antibody by Flow Cytometry.

Detection of GPRC5A in hGPRC5A HEK293/eGFP transfectants by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) Human GPRC5A or (B) irrelevant protein and eGFP, was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of GPRC5A antibody in A549 Human Cell Line antibody by Flow Cytometry.

Detection of GPRC5A in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human GPRC5A Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human GPRC5A and eGFP

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GPRC5A

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

Long Name

G Protein-coupled Receptor, Family C, Group 5, Member A

Alternate Names

RAI3, RAIG1

Entrez Gene IDs

9052 (Human); 232431 (Mouse); 312790 (Rat)

Gene Symbol

GPRC5A

UniProt

Q8NFJ5

Additional GPRC5A Products

Product Documents for Human GPRC5A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GPRC5A Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human GPRC5A Antibody

Customer Reviews for Human GPRC5A Antibody

There are currently no reviews for this product. Be the first to review Human GPRC5A Antibody and earn rewards!

Have you used Human GPRC5A Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies