Human GPRC5A Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Stains human GPRC5A transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2A Clone # 481906
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPRC5A
    Met1-Ser357
    Accession # Q8NFJ5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed A549 human lung carcinoma cell line
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of GPRC5A in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPRC5A

G-protein coupled receptor family C group 5 member A (GPRC5A), also known as Retinoic acid-induced gene 1 protein (RAIG-1), is a member of the G-protein coupled receptor 3 family. It has a molecular weight of approximately 35 kDa and contains seven transmembrane domains. Human GPRC5A shares 76% and 74% aa sequence identity with mouse and rat GPRC5A, respectively. It is expressed at high levels in fetal and adult lung and is also present in fetal kidney and adult placenta, kidney, prostate, testis, ovary, small intestine, colon, stomach, and spinal chord at lower levels.

  • Long Name:
    G Protein-coupled Receptor, Family C, Group 5, Member A
  • Entrez Gene IDs:
    9052 (Human); 232431 (Mouse); 312790 (Rat)
  • Alternate Names:
    G protein-coupled receptor, family C, group 5, member A; GPCR5A; GPRC5A; Orphan G-protein-coupling receptor PEIG-1; RAI3; RAI3G-protein coupled receptor family C group 5 member A; RAIG1; RAIG1RAIG-1; retinoic acid induced 3; retinoic acid responsive; Retinoic acid-induced gene 1 protein; retinoic acid-induced protein 3
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