Human GPRC5B Antibody

Catalog # Availability Size / Price Qty
MAB10253-100
MAB10253-SP
Human GPRC5B Antibody in Flow Cytometry
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Human GPRC5B Antibody Summary

Species Reactivity
Human
Specificity
Detects human GPRC5B in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 575926
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GPRC5B
Accession # Q9NZH0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of GPRC5B  in HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP by Flow  Cytometry. View Larger

Detection of GPRC5B in HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) GPRC5B or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPRC5B Monoclonal Antibody (Catalog # MAB10253) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPRC5B

G-protein coupled receptor family C group 5-member B (GPRC5B) is a retinoic acid-inducible protein that belongs to the seven-transmembrane domain family of receptors. Human GPRC5B shares approximately 85% aa sequence identity with mouse GPRC5B.

Although GPRC5B is considered an "orphan receptor" and its molecular function is not completely understood, it has been implicated in obesity-associated inflammatory signaling in adipocytes and in diet-induced insulin resistance. Consistent with that, CRISPR-Cas9-mediated knock-down of the GPRC5B gene allowed the identification of signaling pathways linking the GPRC5B receptor expression to beta -cell proliferation and apoptosis.

 

Long Name
G Protein-coupled Receptor, Family C, Group 5, Member B
Entrez Gene IDs
51704 (Human); 64297 (Mouse); 293546 (Rat)
Alternate Names
G protein-coupled receptor, family C, group 1, member B; G protein-coupled receptor, family C, group 5, member B; GPRC5B; RAIG2; RAIG-2A-69G12.1; RAIG2G-protein coupled receptor family C group 5 member B; retinoic acid responsive gene protein; Retinoic acid-induced gene 2 protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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