Detection of Human HAND1 by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
HAND1 belongs to the HAND family of conserved basic helix-loop-helix
transcription factors. It is expressed in the heart, ganglia, neural
crest derivatives and extraembryonic tissues. HAND1 plays an essential
role in heart development and is critical for the specification of
extraembryonic tissues including trophoblasts. Human and mouse HAND1
share approximately 92% amino acid sequence homology.
Heart And Neural Crest Derivatives Expressed 1
Entrez Gene IDs:
9421 (Human); 15110 (Mouse); 59112 (Rat)
BHLHA27; bHLHa27heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1; heart and neural crest derivatives expressed 1; Hxt; Thing1