Human HAND1 Antibody

Detection of HAND1 by Immunohistochemistry

Directed differentiation of small ape iPSCs. (A) Schematic overview of direct neurosphere formation and the subsequent neuronal differentiation cultures. (B) Phase-contrast images of representative neurospheres (day 7) and neurons (day 21) derived from small ape iPSCs. (C) Immunofluorescence images of beta III tubulin in day 21 neurons. Nuclei were counterstained with DAPI. Scale bar: 100 μm. (D) Schematic design of limb bud mesoderm differentiation culture. (E) Immunofluorescence images of HAND1 in day 2 lateral plate mesoderm cells and PRRX1 in day 4 limb bud mesoderm cells. Nuclei were counterstained with DAPI. Scale bar: 100 μm. (F) Heatmap showing the expression of genes associated with pluripotency (POU5F1, NANOG), lateral plate mesoderm (HAND1, FOXF1, NKX2.5, ISL1), and limb bud mesoderm (PRRX1, MSX1, PITX1) in iPSCs, day 2 lateral plate mesoderm cells, and day 4 limb bud mesoderm cells determined by quantitative RT-PCR analysis. beta -ACTIN was used as an internal control. (G) Immunofluorescence images of SOX17 in day 2 definitive endoderm cells. Nuclei were counterstained with DAPI. Scale bar: 100 μm. (H) Heatmap showing the expression of genes associated with pluripotency (POU5F1, NANOG) and definitive endoderm (SOX17, FOXA2, GSC, CER1, HHEX, GATA6) in iPSCs and day 2 definitive endoderm cells determined by quantitative RT-PCR analysis. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fcell.2025.1536947/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of HAND1 by Immunocytochemistry/ Immunofluorescence

Pseudotime trajectories of TGF beta -inhibited naïve hPSCs recapitulates early trophectoderm specification in human embryos.(a) Diffusion maps of naïve hPSC 10X scRNA-seq data during the SB time-course, reporting diffusion pseudotime scores. (b) Diffusion map visualisation of naïve cells during the SB time-course reporting the relative expression of additional pluripotency markers KLF4 and DPPA5, TGF beta effector NODAL, and trophoblast markers CDX1, MARCKS, and GATA2. (c) Immunofluorescence microscopy for OCT4 (magenta), HAND1 (yellow), GATA3 (cyan) and DAPI (blue) in H9 NK2 naïve cells following 3 days of SB supplementation to t2iLGö medium. White arrowheads indicate cells that co-express low levels of OCT4 and HAND1 or GATA3. Scale bars: 50 µm. (d) Correlation heatmap between the pseudobulked datasets from all Louvain clusters (A/B/C/D/E) and the identified cell lineages from the cultured human pre-gastrulation embryos (Xiang et al., 2020). CTB: Cytotrophoblast; EVT: Extravillous trophoblast; STB: Syncytiotrophoblast; EPI: Epiblast; ICM: Inner Cell Mass; PrE: Primitive Endoderm, TE: Trophectoderm. (e) Heatmap reporting the expression in the Louvain clusters A, D, and E of the top 20 differentially expressed genes between EPI, ICM, and CTB cell lineages (based on data published by Xiang et al., 2020). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34463252), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of HAND1 by Flow Cytometry

Generation of hPSC-LV-CMs using a two-step approach(A) Depiction of the standard WNT-ON/WNT-OFF protocol; modifications to this protocol (±) and critical feeding steps are indicated. CHIR, CHIR99021; BMP, BMP4; A, activin A; FGF, FGF2; LAA, L-ascorbic acid; IWR1, WNT pathway inhibitor; AGN, AGN193109, a pan-retinoic acid (RA) inhibitor; VitA, vitamin A.(B) Immunostaining micrographs of day 20 hPSC-CMs generated from mesoderm cells exposed to activin A, FGF2, and varying amounts of BMP4 and CHIR, demonstrating the cardiac corridor for making LV-like cardiomyocytes (red box). Scale bar: 100 μm.(C) Flow cytometry plot of day 20 hPSC-LV-CM cultures.(D and E) Day 20 hPSC-CMs generated in the presence or absence of RA signaling (±AGN, ±vitamin A, ±RA).(D) Immunostaining micrographs; scale bar: 100 μm.(E) qRT-PCR analysis.(F and G) Cardiomyocyte quantification in day 20 hPSC-CM cultures differentiated using the standard (Std) or LV protocols. Cells were co-stained with ACTN2, MYL2, and HAND1. DAPI was used to normalize the data.See also Figure S2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37159667), licensed under a CC-BY license. Not internally tested by R&D Systems.