Human HAND1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human HAND1 by Western Blot.
2 Images
Product Details
Citations (6)
Supplemental Products

Human HAND1 Antibody Summary

Species Reactivity
Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human HAND1
Accession # O96004
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
Immersion fixed SH‑SY5Y human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HAND1 antibody by Western Blot. View Larger

Detection of Human HAND1 by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry View Larger

HAND1 in SH‑SY5Y Human Cell Line. HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAND1

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Long Name
Heart And Neural Crest Derivatives Expressed 1
Entrez Gene IDs
9421 (Human); 15110 (Mouse); 59112 (Rat)
Alternate Names
BHLHA27; bHLHa27heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1; HAND1; heart and neural crest derivatives expressed 1; Hxt; Thing1

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Citations for Human HAND1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model
    Authors: M Ohgushi, N Taniyama, A Vandenbon, M Eiraku
    Cell Reports, 2022;39(12):110973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
    Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
    Stem Cell Research, 2022;65(0):102958.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome
    Authors: EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova
    Stem Cell Research, 2022;67(0):103012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis
    Authors: A Mehta, P Lu, BV Taylor, J Charleswor, AL Cook, KP Burdon, AW Hewitt, KM Young
    Stem Cell Research, 2021;57(0):102568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development
    Authors: M Zheng, S Erhardt, D Ai, J Wang
    International Journal of Molecular Sciences, 2021;22(18):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
    Authors: S Chhabra, L Liu, R Goh, X Kong, A Warmflash
    PLoS Biol., 2019;17(10):e3000498.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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