Human HAND1 Antibody Summary
Accession # O96004
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human HAND1 by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HAND1 in SH‑SY5Y Human Cell Line. HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.
Citations for Human HAND1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development
Authors: M Zheng, S Erhardt, D Ai, J Wang
International Journal of Molecular Sciences, 2021;22(18):.
Sample Types: Cell Lysates
Applications: Western Blot
Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis
Authors: A Mehta, P Lu, BV Taylor, J Charleswor, AL Cook, KP Burdon, AW Hewitt, KM Young
Stem Cell Research, 2021;57(0):102568.
Sample Types: Whole Cells
Applications: Flow Cytometry
Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
Authors: S Chhabra, L Liu, R Goh, X Kong, A Warmflash
PLoS Biol., 2019;17(10):e3000498.
Sample Types: Whole Cells
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