Human HAND1 Antibody

Catalog # Availability Size / Price Qty
AF3168
AF3168-SP
Detection of Human HAND1 by Western Blot.
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Product Details
Citations (13)
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Human HAND1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HAND1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HAND2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HAND1
Met1-Gln215
Accession # O96004
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed SH‑SY5Y human neuroblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HAND1 antibody by Western Blot. View Larger

Detection of Human HAND1 by Western Blot. Western blot shows lysates of human heart tissue, human brain (cerebellum) tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HAND1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry View Larger

HAND1 in SH‑SY5Y Human Cell Line. HAND1 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Goat Anti-Human HAND1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3168) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAND1

HAND1 belongs to the HAND family of conserved basic helix-loop-helix transcription factors. It is expressed in the heart, ganglia, neural crest derivatives and extraembryonic tissues. HAND1 plays an essential role in heart development and is critical for the specification of extraembryonic tissues including trophoblasts. Human and mouse HAND1 share approximately 92% amino acid sequence homology.

Long Name
Heart And Neural Crest Derivatives Expressed 1
Entrez Gene IDs
9421 (Human); 15110 (Mouse); 59112 (Rat)
Alternate Names
BHLHA27; bHLHa27heart- and neural crest derivatives-expressed protein 1; Class A basic helix-loop-helix protein 27; eHand; Extraembryonic tissues, heart, autonomic nervous system and neural crestderivatives-expressed protein 1; HAND1; heart and neural crest derivatives expressed 1; Hxt; Thing1

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Citations for Human HAND1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Characterization of an induced pluripotent stem cell line NCHi011-A from a 23-year-old female with Alagille Syndrome harboring a heterozygous JAG1 pathogenic variant
    Authors: Stanberry, I;Cunningham, D;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Population-level antagonism between FGF and BMP signaling steers mesoderm differentiation in embryonic stem cells
    Authors: Marina Gattiglio, Michelle Protzek, Christian Schröter
    Biology Open
  3. Generation of iPSC line NCHi012-A from a patient with Alagille syndrome and heterozygous pathogenic variant in the JAG1 gene
    Authors: Cunningham, D;Stanberry, I;Ye, S;Alonzo, M;Zhao, MT;Garg, V;Lilly, B;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Origin and segregation of the human germline
    Authors: Aracely Castillo-Venzor, Christopher A Penfold, Michael D Morgan, Walfred WC Tang, Toshihiro Kobayashi, Frederick CK Wong et al.
    Life Science Alliance
  5. Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay
    Authors: Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas, Celine M. Roelse, Sanne Hillenius, Talia Van Der Helm et al.
    Cell Reports Methods
  6. The time integral of BMP signaling determines fate in a stem cell model for early human development
    Authors: S Teague, G Primavera, B Chen, E Freeburne, H Khan, K Jo, C Johnson, I Heemskerk
    bioRxiv : the preprint server for biology, 2023-04-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome
    Authors: EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova
    Stem Cell Research, 2022-12-30;67(0):103012.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  8. Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)
    Authors: H Lin, SQ Ye, ZH Xu, JS Penaloza, M Aljuhani, T Vetter, MT Zhao, KL McBride
    Stem Cell Research, 2022-10-31;65(0):102958.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model
    Authors: M Ohgushi, N Taniyama, A Vandenbon, M Eiraku
    Cell Reports, 2022-06-21;39(12):110973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  10. A stress-reduced passaging technique improves the viability of human pluripotent cells
    Authors: Kazutoshi Takahashi, Chikako Okubo, Michiko Nakamura, Mio Iwasaki, Yuka Kawahara, Tsuyoshi Tabata et al.
    Cell Reports Methods
  11. Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis
    Authors: A Mehta, P Lu, BV Taylor, J Charleswor, AL Cook, KP Burdon, AW Hewitt, KM Young
    Stem Cell Research, 2021-10-12;57(0):102568.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  12. Bmp Signaling Regulates Hand1 in a Dose-Dependent Manner during Heart Development
    Authors: M Zheng, S Erhardt, D Ai, J Wang
    International Journal of Molecular Sciences, 2021-09-11;22(18):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
    Authors: S Chhabra, L Liu, R Goh, X Kong, A Warmflash
    PLoS Biol., 2019-10-15;17(10):e3000498.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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