Human HCN4 Antibody

Catalog # Availability Size / Price Qty
HCN4 in BG01V Human Embryonic Stem Cells.
1 Image
Product Details
Citations (1)
Supplemental Products

Human HCN4 Antibody Summary

Species Reactivity
Detects human HCN4 in ELISA
Monoclonal Mouse IgG1 Clone # 880904
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HCN4
Accession # Q9Y3Q4
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HCN4 antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

HCN4 in BG01V Human Embryonic Stem Cells. HCN4 was detected in immersion fixed BG01V human embryonic stem cells differentiated to cardiac cells using Mouse Anti-Human HCN4 Monoclonal Antibody (Catalog # MAB8138) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HCN4

HCN4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) is a 120-160 kDa, 1203 amino acid (aa) multipass plasma membrane protein that is a member of the HCN family of potassium channel proteins. It is mainly expressed in cardiac myocytes, thalamus and testis. HCN4 is important for pacemaker function in the heart, and rhythmic activity in the thalamus. Specific mutations can cause Sick Sinus Syndrome 2 (SSS2), a cardiac sinus node malfunction, or Brugada syndrome 8 (BRGDA8), a tachyarrhythmia syndrome. Within aa 1084-1203, human HCN4 shares 92% and 93% aa sequence identity with mouse and rat HCN4, respectively.

Long Name
Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4
Entrez Gene IDs
10021 (Human); 330953 (Mouse); 59266 (Rat)
Alternate Names
HCN4; hyperpolarization activated cyclic nucleotide-gated cation channel 4; hyperpolarization activated cyclic nucleotide-gated potassium channel 4; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4; SSS2

Product Datasheets

You must select a language.


Citation for Human HCN4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Human ISL1+ Ventricular Progenitors Self-Assemble into an In Vivo Functional Heart Patch and Preserve Cardiac Function Post Infarction
    Authors: Kylie S. Foo, Miia L. Lehtinen, Chuen Yan Leung, Xiaojun Lian, Jiejia Xu, Wendy Keung et al.
    Molecular Therapy


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human HCN4 Antibody

There are currently no reviews for this product. Be the first to review Human HCN4 Antibody and earn rewards!

Have you used Human HCN4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review