Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

Catalog # Availability Size / Price Qty
MAB6085
MAB6085-SP
Heparan Sulfate 3-O-Sulfotransferase 4/ HS3ST4 in Human Brain.
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Product Details
Citations (2)
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Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HS3ST3B1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 712010
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4
Gly184-Lys456
Accession # Q9Y661
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human HS3ST4 (Catalog # 6085‑ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Heparan Sulfate 3-O-Sulfotransferase 4/ HS3ST4 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Heparan Sulfate 3-O-Sulfotransferase 4/ HS3ST4 in Human Brain. Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Monoclonal Antibody (Catalog # MAB6085) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5).

References
  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
  6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
  7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
  8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.
Entrez Gene IDs
9951 (Human)
Alternate Names
30ST4; 3OST4 heparan sulfate 3-O-sulfotransferase-4; 3-OST-4; EC 2.8.2; EC 2.8.2.23; h3-OST-4; heparan sulfate (glucosamine) 3-O-sulfotransferase 4; Heparan sulfate 3-O-sulfotransferase 4; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4; heparan sulfate glucosamine 3-O-sulfotransferase 4; HS3ST4

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Citations for Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cancer cells induce immune escape via glycocalyx changes controlled by the telomeric protein TRF 2
    Authors: Julien Cherfils‐Vicini, Charlene Iltis, Ludovic Cervera, Sabrina Pisano, Olivier Croce, Nori Sadouni et al.
    The EMBO Journal
  2. TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Authors: Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
    Nat Cell Biol, 2013-06-23;15(7):818-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot

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