Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HS3ST3B1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 712010
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4
    Gly184-Lys456
    Accession # Q9Y661
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Heparan Sulfate 3-O-Sulfotransferase 4/
HS3ST4 in Human Brain.
Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Monoclonal Antibody (Catalog # MAB6085) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4
Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5).
  • References:
    1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
    2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
    3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
    4. Xu, D. et al. (2005) Biochem. J. 386:451.
    5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
    6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
    7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
    8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.
  • Entrez Gene IDs:
    9951 (Human)
  • Alternate Names:
    30ST4; 3OST4 heparan sulfate 3-O-sulfotransferase-4; 3-OST-4; EC 2.8.2; EC 2.8.2.23; h3-OST-4; heparan sulfate (glucosamine) 3-O-sulfotransferase 4; Heparan sulfate 3-O-sulfotransferase 4; Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4; heparan sulfate glucosamine 3-O-sulfotransferase 4; HS3ST4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Authors: Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke C, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lepinasse F, Marvel J, Verhoeyen E, Cosset F, Peeper D, Smyth M, Londono-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec J, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
    Nat Cell Biol, 2013;15(7):818-28.
    Species: Human
    Sample Type: Whole Cells
    Application: WB Ctrl
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