Human HES-4 Antibody

Catalog # Availability Size / Price Qty
MAB3600
MAB3600-SP
Detection of Human HES‑4 by Western Blot.
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Human HES-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HES-4 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 455237
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HES-4
Val94-Arg221
Accession # Q9HCC6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HES‑4 antibody by Western Blot. View Larger

Detection of Human HES‑4 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human HES-4 Monoclonal Antibody (Catalog # MAB3600) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HES-4 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-4

The Notch pathway maintains embryonic stem cells by up‑regulation of HES and Hey basic helix-loop-helix family members, which repress tissue specific expression. HES-4 is up‑regulated in gastric and colorectal cancers. It shares high homology with HES-1, HES-2, and HES-3.

Long Name
Hairy And Enhancer of Split 4
Entrez Gene IDs
57801 (Human)
Alternate Names
bHLH factor Hes4; BHLHB42; bHLHb42transcription factor HES-4; Class B basic helix-loop-helix protein 42; hairy and enhancer of split 4 (Drosophila); Hairy and enhancer of split 4; HES4; HES-4; hHES4

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Human HES-4 Antibody
By Anonymous on 01/18/2022
Application: WB Sample Tested: T cells Species: Human