Human HES-4 Antibody Summary
Accession # Q9HCC6
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human HES‑4 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human HES-4 Monoclonal Antibody (Catalog # MAB3600) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HES-4 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The Notch pathway maintains embryonic stem cells by up‑regulation of HES and Hey basic helix-loop-helix family members, which repress tissue specific expression. HES-4 is up‑regulated in gastric and colorectal cancers. It shares high homology with HES-1, HES-2, and HES-3.
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