Human HGF Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human HGF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse HGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF (R&D Systems, Catalog # 294-HG)
    Gln32-Ser728
    Accession # P14210
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human HGF (Catalog # 294-HG)
  • Neutralization
    Measured by its ability to neutralize HGF-induced IL-11 secretion in the Saos‑2 human osteosarcoma cell line. Hjertner, O. et al. (1999) Blood 94:3883. The Neutralization Dose (ND50) is typically <2 μg/mL in the presence of 3 ng/mL Recombinant Human HGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HGN) induces IL-11 secretion in the Saos-2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA). The ND50 is typically 2 μg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

  • Long Name:
    Hepatocyte Growth Factor
  • Entrez Gene IDs:
    3082 (Human); 15234 (Mouse); 403441 (Canine)
  • Alternate Names:
    deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Hepatocyte growth factor produced in lung fibroblasts enhances non-small cell lung cancer cell survival and tumor progression
    Authors: N Kanaji, M Yokohira, Y Nakano-Nar, N Watanabe, K Imaida, N Kadowaki, S Bandoh
    Respir. Res., 2017;18(1):118.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. c-Met-mediated endothelial plasticity drives aberrant vascularization and chemoresistance in glioblastoma
    Authors: M Huang, T Liu, P Ma, RA Mitteer, Z Zhang, HJ Kim, E Yeo, D Zhang, P Cai, C Li, L Zhang, B Zhao, L Roccogrand, DM O'Rourke, N Dahmane, Y Gong, C Koumenis, Y Fan
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
    Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009;296(4):C922-9.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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