Human HIF-2 alpha /EPAS1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB2997-100
MAB2997-SP
Human HIF-2 alpha /EPAS1 Antibody in Western Blot
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Human HIF-2 alpha /EPAS1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HIF-2 alpha /EPAS1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2444A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human HIF-2 alpha /EPAS1
Ser543-Thr870
Accession # Q99814
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human HIF‑2 alpha /EPAS1 by Western Blot. View Larger

Detection of Human HIF‑2 alpha /EPAS1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF‑2 alpha /EPAS1 Monoclonal Antibody (Catalog # MAB2997) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIF‑2 alpha /EPAS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HIF‑2 alpha /EPAS1  in HepG2 Human Cell Line. View Larger

HIF‑2 alpha /EPAS1 in HepG2 Human Cell Line. HIF‑2 alpha /EPAS1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with DFO (left panel; positive stain) or untreated (right panel; negative stain) using Rabbit Anti-Human HIF‑2 alpha /EPAS1 Monoclonal Antibody (Catalog # MAB2997) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in DFO treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.

Long Name
Hypoxia-inducible Transcription Factor 2 alpha
Entrez Gene IDs
2034 (Human)
Alternate Names
Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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