Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2444A
View all HIF-2 alpha/EPAS1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HIF-2 alpha /EPAS1
Ser543-Thr870
Accession # Q99814

Specificity

Detects human HIF-2 alpha /EPAS1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human HIF‑2 alpha /EPAS1 Antibody

Detection of Human HIF-2a/EPAS1 antibody by Western Blot.

Detection of Human HIF‑2 alpha /EPAS1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human HIF-2a/EPAS1 Monoclonal Antibody (Catalog # MAB2997) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIF-2a/EPAS1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HIF-2a/EPAS1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

HIF‑2 alpha /EPAS1 in HepG2 Human Cell Line.

HIF-2a/EPAS1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with DFO (left panel; positive stain) or untreated (right panel; negative stain) using Rabbit Anti-Human HIF-2a/EPAS1 Monoclonal Antibody (Catalog # MAB2997) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in DFO treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human HIF‑2 alpha /EPAS1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line treated with DFO

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with DFO

Reviewed Applications

Read 1 review rated 5 using MAB2997 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIF-2 alpha/EPAS1

The hypoxia-inducible transcription factor 2 alpha (HIF-2 alpha ) is stabilized in hypoxic tissue and, similarly to HIF-1 alpha, complexes with Aryl hydrocarbon receptor nuclear translocator (ARNT). Both the HIF-1 and HIF-2 complexes bind hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. HIF-1 and HIF-2 do not appear completely redundant, although specific functions are only beginning to be elucidated. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the potentially broad impact of gene regulation by both HIF-1 alpha and HIF-2 alpha.

Long Name

Hypoxia-inducible Transcription Factor 2 alpha

Alternate Names

EPAS1, HIF 2A, HIF2 alpha, HIF2A, HLF, MOP2

Entrez Gene IDs

2034 (Human); 13819 (Mouse)

Gene Symbol

EPAS1

UniProt

Q99814

Additional HIF-2 alpha/EPAS1 Products

Product Documents for Human HIF‑2 alpha /EPAS1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HIF‑2 alpha /EPAS1 Antibody

For research use only

Customer Reviews for Human HIF‑2 alpha /EPAS1 Antibody (1)

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  • Human HIF-2 alpha /EPAS1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: MCF-7 cells
    Species: Human
    Verified Customer | Posted 11/23/2021
    Human HIF‑2 alpha /EPAS1 Antibody MAB2997

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Protocols

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