Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 410219
View all HIN-1/SCGB3A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HIN‑1/SCGB3A1
Phe21-Gly104
Accession # Q96QR1

Specificity

Detects human HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant mouse HIN-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human HIN‑1/SCGB3A1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed Y-79 human retinoblastoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human HIN-1/SCGB3A1

Reviewed Applications

Read 1 review rated 5 using MAB27901 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HIN-1/SCGB3A1

High in Normal-1 (HIN-1), also known as Secretoglobulin 3A1 (SCGB3A1), PnSP-2, LuLeu-2, and UGRP-2 is an 8 kDa member of the Secretoglobulin family of molecules. It is synthesized as a 104 aa precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N-linked glycosylation sites. Based on other members of the family, HIN-1 is likely to exist as a disulfide-linked homodimer. The molecule is found in mammary and upper airway epithelium. Mature mouse HIN-1 shares 60% aa sequence identity with mature human HIN-1.

Long Name

High in Normal 1

Alternate Names

HIN1, PnSP-2, SCGB3A1, UGRP2

Entrez Gene IDs

92304 (Human); 73945 (Mouse)

Gene Symbol

SCGB3A1

UniProt

Q96QR1

Additional HIN-1/SCGB3A1 Products

Product Documents for Human HIN‑1/SCGB3A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human HIN‑1/SCGB3A1 Antibody

For research use only

Citations for Human HIN‑1/SCGB3A1 Antibody

Customer Reviews for Human HIN‑1/SCGB3A1 Antibody (1)

5 out of 5
1 Customer Rating
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100%
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1 Stars
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  • Human HIN-1/SCGB3A1 Antibody
    Name: avinash waghray
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: AIRWAYS
    Species: Human
    Verified Customer | Posted 02/26/2019
    Staining was done on human airways after citrate antigen retrieval. OCT sections were used

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Protocols

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