Human HIPK1 Antibody

Catalog # Availability Size / Price Qty
AF5617
AF5617-SP
Detection of Human HIPK1 by Western Blot.
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Human HIPK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human HIPK1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HIPK1
Gln701-Ser900
Accession # Q86Z02
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human HIPK1 antibody by Western Blot. View Larger

Detection of Human HIPK1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human HIPK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIPK1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIPK1

HIPK1 (Homeodomain-interacting protein kinase 1) is a 120‑130 kDa member of the HIPK subfamily, CMGC Ser/Thr family of protein kinases. It is ubiquitously expressed, and plays a role in transcriptional regulation and signal transduction. Human HIPK1 is 1210 amino acids (aa) in length. It contains a protein kinase domain (aa 190‑518) that phosphorylates itself as well as Daxx and p53, an interaction region between aa 581‑847, a PEST sequence (aa 889‑973) and a YH domain (aa 1066‑1209) that recruits CtBP. Four different splice variants have been identified. Over aa 701‑900, human and mouse HIPK1 share 97% aa identity.

Long Name
Homeodomain Interacting Protein Kinase 1
Entrez Gene IDs
204851 (Human); 15257 (Mouse); 365895 (Rat)
Alternate Names
EC 2.7.11.1; HIPK1; homeodomain interacting protein kinase 1; homeodomain interacting protein kinase 1-like protein; homeodomain-interacting protein kinase 1; KIAA0630MGC26642; MGC33446; MGC33548; Myak; Nbak2; nuclear body associated kinase 2b

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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