Human HNF-4 alpha/NR2A1 Antibody

Isoforms 7-9
Catalog # Availability Size / Price Qty
PP-H6939-00
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews

Human HNF-4 alpha/NR2A1 Antibody Summary

Species Reactivity
Human
Source
Monoclonal Mouse IgG1 Clone # H6939
Formulation
A liquid formulation in physiologic saline with 0.1% NaN3.

Applications

Recommended Concentration
Sample
Western Blot
Please refer to the PDF datasheet for specifications.
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human large intestine
Immunoprecipitation
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 alpha/NR2A1

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name
Hepatocyte Nuclear Factor-4, alpha
Entrez Gene IDs
3172 (Human)
Alternate Names
FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; hepatocyte nuclear factor 4-alpha; HNF4 alpha; HNF-4 alpha; HNF4A; HNF4a7; HNF-4-alpha; HNF4alpha10/11/12; HNF4HNF4a8; MODY; MODY1; NR2A1; NR2A1HNF4a9; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF14; TCF-14; TCF14HNF4alpha; Transcription factor 14; Transcription factor HNF-4; transcription factor-14

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Citations for Human HNF-4 alpha/NR2A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma
    Authors: Z Wang, X Zhang, X Tian, Y Yang, L Ma, J Wang, Y Yu
    Oncology reports, 2021-04-13;45(6):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  2. Ferroptosis is governed by differential regulation of transcription in liver cancer
    Authors: X Zhang, L Du, Y Qiao, X Zhang, W Zheng, Q Wu, Y Chen, G Zhu, Y Liu, Z Bian, S Guo, Y Yang, L Ma, Y Yu, Q Pan, F Sun, J Wang
    Redox Biol, 2019-05-10;24(0):101211.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP, Immunoprecipitation, Proximity Ligation Assay
  3. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
    Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
    Dev. Cell, 2018-03-26;44(6):679-693.e5.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors
    Authors: PB Ancey, S Ecsedi, MP Lambert, FR Talukdar, MP Cros, D Glaise, DM Narvaez, V Chauvet, Z Herceg, A Corlu, H Hernandez-
    Stem Cell Reports, 2017-06-22;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Mutant IDH inhibits HNF-4alpha to block hepatocyte differentiation and promote biliary cancer.
    Authors: Saha S, Parachoniak C, Ghanta K, Fitamant J, Ross K, Najem M, Gurumurthy S, Akbay E, Sia D, Cornella H, Miltiadous O, Walesky C, Deshpande V, Zhu A, Hezel A, Yen K, Straley K, Travins J, Popovici-Muller J, Gliser C, Ferrone C, Apte U, Llovet J, Wong K, Ramaswamy S, Bardeesy N
    Nature, 2014-07-02;513(7516):110-4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Novel P2 promoter-derived HNF4alpha isoforms with different N-terminus generated by alternate exon insertion.
    Authors: Huang J, Levitsky LL, Rhoads DB
    Exp. Cell Res., 2009-04-15;315(7):1200-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Mobility Shift Assay

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