Human ICOS Antibody

Catalog # Availability Size / Price Qty
MAB6975
MAB6975-SP
Detection of ICOS in Human PBMCs by Flow Cytometry.
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Human ICOS Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICOS in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 669222
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human ICOS and mouse myeloma cell line NS0-derived recombinant human ICOS cocktail
Glu21-Phe141
Accession # Q9Y6W8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of ICOS in Human PBMCs by Flow Cytometry. View Larger

Detection of ICOS in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 50 ng/mL PMA and 500 ng/mL calcium ionomycin for 24 hours were stained with Mouse Anti-Human ICOS Monoclonal Antibody (Catalog # MAB6975) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3 epsilon PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB002).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICOS

Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References
  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Entrez Gene IDs
29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)
Alternate Names
Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; ICOS; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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