Human IFN-alpha 2/IFNA2 Antibody Summary
Accession # P01563
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ alpha 2/IFNA2 in Human PBMCs. IFN‑ alpha 2/IFNA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IFN‑ alpha 2/IFNA2 Monoclonal Antibody (Catalog # MAB93455) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha 2/IFNA2
Interferon-alpha 2 (IFNA2), also known as leukocyte interferon, is a type I interferon produced by macrophages, CD8 resting T cells, tonsillar NK cells, germinal center B cells, as well as epithelial cells, tumor cells and melanocytes. IFN alpha has at least nine different isoforms.
IFNA2 is a ligand for a cell surface receptor with two subunits, IFN-alpha R2 (ligand binding) and IFN-alpha R1 (ligand binding and signal transduction). IFNA2 has both antiviral and immunomodulatory activities on target cells. Intranasal administration has been shown to reduce viral load in subjects with the common cold. While IFNA2 is a prognostic factor for hepatocellular carcinoma, Interferon alpha 2b, has been used to treat melanoma, renal cell carcinoma, chronic myelogenous leukemia and hepatitis C.
- Chen, Zy et al. (2014) Br. J. Cancer 110:733.
- Burchert, A. et al. (2015) Leukemia 29:1331.
- Perrakis, A. et al. (2011) Transplant Proc. 43:3824.
- Akaza, H. et al. (2011) Jpn. J. Clin. Oncol. 41:1023.
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