Human IFN‑ alpha Antibody

Name: Human IFN‑ alpha Antibody
Brand: R&D Systems
SKU: 21125-1
Price: 369 USD
Availability: InStock

R&D Systems | Catalog # 21125-1

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # MMHA-6

View all IFN-alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Interferon Alpha D

Specificity

Neutralizes human interferon alpha; binds to human interferon alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Endotoxin Level

<1.0 EU per 1 μg of the antibody by the LAL method.

Applications for Human IFN‑ alpha Antibody

Application

Recommended Usage

ELISA

Optimal dilutions should be determined by each laboratory for each application.

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN- alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol . 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN- alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, the product may be stored at -20°C for short-term use ( ≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.