Human IFN-beta Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IFN‑ beta in direct ELISAs.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 2036A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human IFN‑ beta
    Met1-Asn187
    Accession # P01574
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IFN‑ beta in A549 Human Cell Line. IFN‑ beta was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human IFN‑ beta Monoclonal Antibody (Catalog # MAB8142) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.

  • Long Name:
    Interferon beta
  • Entrez Gene IDs:
    3456 (Human); 15977 (Mouse); 24481 (Rat)
  • Alternate Names:
    Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956
Related Research Areas
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Isotype Controls
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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Rabbit IgG Fluorescein-conjugated Antibody

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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 3
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rabbit IgG Antibody

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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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