Human IFN-beta Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8142-SP
MAB8142-100

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IFN‑ beta  in A549 Human Cell Line.
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Product Details
Citations (1)
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Human IFN-beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ beta in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2036A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IFN‑ beta
Met1-Asn187
Accession # P01574
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µL/106 cells
M1 macrophages treated with 50 ng/mL each of rhGM-CSF (Catalog # 215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (Catalog # 285-IF) and restimulated with monensin for 3 hours (3uM)
Immunocytochemistry
1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IFN-beta  antibody in A549 Human Cell Line by Immunocytochemistry (ICC). View Larger

IFN‑ beta in A549 Human Cell Line. IFN-beta was detected in immersion fixed A549 human lung carcinoma cell line using Rabbit Anti-Human IFN-beta Monoclonal Antibody (Catalog # MAB8142) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow Cytometry View Larger

Detection of IFN‑ beta in M1 macrophages by Flow Cytometry. M1 macrophages treated with 50 ng/mL each of rhGM-CSF (215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (285-IF) and restimulated with monensin for 3 hours (3uM) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Rabbit Anti-Human IFN‑ beta Monoclonal Antibody (Catalog # MAB8142) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

Product Datasheets

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Product Specific Notices

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Citation for Human IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Antagonism between viral infection and innate immunity at the single-cell level
    Authors: Grabowski, F;Kocha?czyk, M;Korwek, Z;Czerkies, M;Prus, W;Lipniacki, T;
    PLoS pathogens
    Applications: IF

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