Human IL-10 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB91841-100
MAB91841-SP
Human IL‑10 ELISA Standard Curve.
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Human IL-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-10 or recombinant rat IL-10 is observed.
Source
Recombinant Monoclonal Mouse IgG Clone # 21204R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2172).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-10 DuoSet ELISA Kit (Catalog # DY217B) for convenient development of a sandwich ELISA or the Human IL-10 Quantikine ELISA Kit (Catalog # D1000B) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human IL‑10 ELISA Standard Curve. Recombinant Human IL-10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB91841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Human IL-10 Antibody
By Anonymous on 08/10/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human