Human IL-10 Antibody Summary
Accession # P22301
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2172).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-10 DuoSet ELISA Kit (Catalog # DY217B) for convenient development of a sandwich ELISA or the Human IL-10 Quantikine ELISA Kit (Catalog # D1000B) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human IL‑10 ELISA Standard Curve. Recombinant Human IL-10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB91841) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
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