Interleukin-10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry-Paraffin, Array Development, Bioassay, ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 127107
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Ser19-Asn178
Accession # P22301
Specificity
Detects human IL-10 in ELISAs. In sandwich immunoassays, less than 5% cross-reactivity with recombinant equine IL-10 is observed and less than 0.5% cross-reactivity with recombinant canine IL-10, recombinant mouse IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant cotton rat IL-10, and recombinant feline IL-10 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IL‑10 Antibody
Detection of Human IL‑10 by Western Blot.
Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2172) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IL‑10 Antibody
Application
Recommended Usage
Western Blot
5 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Sample: HEK293 human embryonic kidney cell line transfected with human IL-10
Human IL-10 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 7 reviews rated 4.9 using MAB2172 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-10
Long Name
Interleukin 10
Alternate Names
CSIF, GVHDS, IL10, IL10A, TGIF
Entrez Gene IDs
Gene Symbol
IL10
UniProt
Additional IL-10 Products
Product Documents for Human IL‑10 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑10 Antibody
For research use only
Related Research Areas
Citations for Human IL‑10 Antibody
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Customer Reviews for Human IL‑10 Antibody (7)
4.9 out of 5
7 Customer Ratings
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Application: ImmunoprecipitationSample Tested: SerumSpecies: HumanVerified Customer | Posted 09/06/2018
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 09/05/2018
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Application: Western BlotSample Tested: Purified proteinSpecies: HumanVerified Customer | Posted 09/05/2018
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Application: Western BlotSample Tested: Purified proteinSpecies: E. coliVerified Customer | Posted 08/10/2018
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 07/17/2018
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Application: Gel ShiftSample Tested: 786-O human renal cell adenocarcinoma cell lineSpecies: HumanVerified Customer | Posted 04/05/2018
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Application: Siteclick antibody biotinylationSample Tested: Monocyte-derived immature dendritic cellsSpecies: MouseVerified Customer | Posted 10/27/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
IL-2 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Innate Lymphoid Cell Differentiation Pathways
Jak/STAT Signaling Pathway
