Human IL-10 R beta Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human
    (rh) IL‑20 R alpha, rhIL-10 R, and rhIL-20 R beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
    Met20-Ser220
    Accession # Q08334
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.20 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood monocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta  Antibody. Recombinant Human IL‑10 (Catalog # 217-IL) inhibits IL‑1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‑1 beta /IL‑1F2 Quantikine ELISA Kit (Catalog # DLB50).
IL‑1 beta secretion inhibited by Recombinant Human IL‑10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF874). The ND50 is typically 2-6 µg/mL in the presence of LPS (0.25 ng/mL).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-10 R beta

IL-10, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) residue type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment, and a short 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two
IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously.
IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

  • References:
    1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
    2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
    3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
    4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
    5. Lutfalla, G. et al. (1993) Genomics 16:366.
    6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  • Long Name:
    Interleukin 10 Receptor beta
  • Entrez Gene IDs:
    3588 (Human); 16155 (Mouse)
  • Alternate Names:
    CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Trisomy 21 consistently activates the interferon response
    Elife, 2016;5(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. HCV infection selectively impairs type I but not type III IFN signaling.
    Authors: Chandra P, Bao L, Song K, Aboulnasr F, Baker D, Shores N, Wimley W, Liu S, Hagedorn C, Fuchs S, Wu T, Balart L, Dash S
    Am J Pathol, 2014;184(1):214-29.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
    Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
    Genes Immun., 2009;10(8):702-14.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Biological activity of interleukins-28 and -29: comparison with type I interferons.
    Authors: Meager A, Visvalingam K, Dilger P, Bryan D, Wadhwa M
    Cytokine, 2005;31(2):109-18.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2.
    Authors: Sheikh F, Baurin VV, Lewis-Antes A, Shah NK, Smirnov SV, Anantha S, Dickensheets H, Dumoutier L, Renauld JC, Zdanov A, Donnelly RP, Kotenko SV
    J. Immunol., 2004;172(4):2006-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Abnormal interleukin 10Ralpha expression contributes to the maintenance of elevated cyclooxygenase-2 in non-small cell lung cancer cells.
    Authors: Heuze-Vourc&amp;amp;apos;h N
    Cancer Res., 2003;63(4):766-70.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. IL-28, IL-29 and their class II cytokine receptor IL-28R.
    Authors: Sheppard P, Kindsvogel W, Xu W, Henderson K, Schlutsmeyer S, Whitmore TE, Kuestner R, Garrigues U, Birks C, Roraback J, Ostrander C, Dong D, Shin J, Presnell S, Fox B, Haldeman B, Cooper E, Taft D, Gilbert T, Grant FJ, Tackett M, Krivan W, McKnight G, Clegg C, Foster D, Klucher KM
    Nat. Immunol., 2002;4(1):63-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
Isotype Controls
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Normal Goat IgG Control

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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