Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-10 R beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
Met20-Ser220
Accession # Q08334

Specificity

Detects human IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human(rh) IL‑20 R alpha, rhIL-10 R, and rhIL-20 R beta is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.20 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL‑10 R beta Antibody

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta Antibody.

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta Antibody.

Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF874). The ND50 is typically 2-6 µg/mL in the presence of LPS (0.25 ng/mL).

Applications for Human IL‑10 R beta Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)

Neutralization

Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10 R beta

IL-10, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) residue type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment, and a short 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two
IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously.
IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

References

  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
  3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
  5. Lutfalla, G. et al. (1993) Genomics 16:366.
  6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

Long Name

Interleukin 10 Receptor beta

Alternate Names

CDw210b, CRFB4, D21S58, IL-10R2, IL-10Rb, IL10R beta, IL10RB

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Gene Symbol

IL10RB

UniProt

Q08334

Additional IL-10 R beta Products

Product Documents for Human IL‑10 R beta Antibody

Certificate of Analysis

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Product Specific Notices for Human IL‑10 R beta Antibody

For research use only

Citations for Human IL‑10 R beta Antibody

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